产生VIM-1的Enterobacter asburiae具有来自废水的移动胆固醇抗性基因
Iva Sukkar1,2, Adam Valcek1,3,4, Monika Dolejska5,6,7,8
1Central European Institute of Technology, University of Veterinary Sciences Brno, Brno, Czech Republic.
BMC genomics
|September 19, 2024
概括
废水分析显示,产生碳酶的Enterobacter asburiae携带了像VIM-1和mcr-10这样的耐药基因. 这突出了废水的重点.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 基因组学就是基因组学.
背景情况:
- 污水是对抗微生物耐药性 (AMR) 传播的重要贡献者,影响人类和动物的健康.
- 这项研究调查了废水作为碳烯酶产生肠道细菌 (CPE) 的潜在环境来源.
研究的目的:
- 在医院和市政废水和河水中识别产生卡巴酶的肠杆菌.
- 描述耐药性的遗传基础,包括碳烯酶和胆固醇抗性基因.
- 评估Enterobacter asburiae在抗生素耐药性传播中的作用.
主要方法:
- 从废水和河流样本中选择性培养大肠杆菌,在补充美罗的介质上.
- 聚合酶链反应 (PCR) 用于检测碳酶编码基因.
- 抗菌敏感性测试,碳酶生产测定和全基因组测序 (短读和长读).
主要成果:
- 在医院废水,市政废水和河流样本中发现了三种产生VIM的Enterobacter asburiae分离物.
- 在各种等离子体上发现了blaVIM-1基因,在分离物中检测到胆固醇抗性基因 (mcr-10,mcr-9).
- 对公共E. asburiae基因组的分析揭示了碳酶基因的频繁携带,强调了该物种在AMR中的作用.
结论:
- 医院和社区的废水在传播多药耐药性病原体方面发挥着至关重要的作用.
- 肠杆菌 (Enterobacter asburiae) 是环境中碳烯酶和素抗性基因的重要储存体.
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