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来自Ipomoea nil的甜病毒2的新分离物:基于完整基因组的分子特征,代码使用偏差和家族遗传分析

Kun-Jiang Wei¹, Ai-Ming Jiang¹, Shuo Jiang¹

¹Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety, Agricultural College, Guangxi University, Nanning, 520004, China.

Virology journal

|September 19, 2024

概括

这项研究在中国发现了一种新的甜病毒2 (SPV2) 隔离物,SPV2-LN. 基因分析显示,它与其他中国SPV2菌株有着密切的关系,有助于了解病毒的进化和传播.

科学领域:

植物病毒学植物病毒学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

病毒性疾病导致了全球甜的大量作物损失.
超过30种病毒感染了甜,甜病毒2 (SPV2) 是主要的病原体.
SPV2属于Potyviridae家族中的Potyvirus属. SPV2属于Potyviridae家族中的Potyvirus属. SPV2的原始原始原始原始原始原始原始原始原始原始原始原始原始原始.

研究的目的:

鉴定和描述一种新型的SPV2从中国的Ipomoea nil中分离出来.
为了分析完整的基因组序列,编码子使用,分化,重组和SPV2-LN分离的选择性压力.

主要方法:

在Ipomoea中检测到SPV2分离物 (SPV2-LN).
测序小RNAs,RT-PCR和RACE放大用于完整的基因组确定.
科登使用,遗传学,重组和选择性压力分析.

主要成果:

该SPV2-LN基因组长达10,606个核酸,编码3425个氨基酸.
蛋白质P3和P3N-PIPO显示出最高的变异性,而P1是最保守的.
遗传学分析将SPV2-LN与中国分离物 (yu-17-47) 结合在一起;没有检测到主要的重组地点.
对大多数SPV2蛋白质观察到负选择,除了P1N-PISPO.

结论:

在中国的Ipomoea nil中发现了一种新的SPV2分离物SPV2-LN.
SPV2-LN与中国甜分离物 (yu-17-47) 的高序列相同.
这些发现为了解SPV2遗传演变和病毒传播提供了基础.

关键词:

在Codon的使用中,存在偏见.完整的基因组序列.人类遗传学分析选择性的压力选择性压力.甜病毒2 甜病毒2

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