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一个Vip3Af突变使得对广泛的类昆虫害虫具有很高的抵抗力

Yajie Sun^1,2, Pan Yang¹, Mingjun She¹

¹Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.

Pest management science

|September 20, 2024

概括

一种新的突变植物性杀虫蛋白 (Vip3Af-T686R) 显示出对关键类虫害的杀虫活性增强. 这种改进的Vip3A突变为玉米等作物提供了更广泛的害虫防治.

科学领域:

农业昆虫学农业昆虫学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

来自Bacillus thuringiensis (Bt) 的植物杀虫蛋白 (Vip3) 对于控制类害虫至关重要.
现有的Vip3蛋白对像秋军虫 (FAW) 这样的夜虫害有很高的有效性,但对其他如亚洲玉米虫 (ACB) 的活性有限.

研究的目的:

为了设计一种具有更广泛杀虫谱和增强活性的Vip3A突变物.
评估工程Vip3A突变对主要类虫害的疗效.

主要方法:

通过局部导向的突变发生被用来制造Vip3Af-T686R,这是Vip3Af.T686R的一个单氨基酸突变.
在体外蛋白质分解活性测试中,使用各种昆虫害虫的中肠汁进行了测试.
产生了表达突变Vip3Af的转基因玉米,并对昆虫耐药性进行了测试.

主要成果:

突变Vip3Af-T686R对ACB和东方军 (OAW) 的杀虫活性显著增加,同时对FAW,棉花 (CBW) 和大米茎 (RSB) 保持高效.
在ACB和OAW的中肠中观察到突变蛋白质的增强的蛋白质分解激活效率.
表达Vip3Af-T686R的转基因玉米系对包括ACB,OAW,FAW,甜菜军虫 (BAW) 和CBW在内的多种类虫害表现出强大的抵抗力.

结论:

Vip3Af-T686R代表了一种有希望的Vip3A突变,用于开发具有对虫虫害的广泛耐药性的转基因作物.
这种工程蛋白质可以显著改善农业中的害虫管理策略.

关键词:

在Vip3Af中使用Vip3Af.突变蛋白质是一种突变蛋白质.害虫控制害虫控制害虫控制害虫控制转基因玉米转基因玉米

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