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商业带启发的基于CRISPR的单芯片用于多重检测呼吸系统病毒
Hong Zhang1,2, Xiaolin Hu2,3, Xudong Bao2,3
1Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandong, 250033, China.
Small methods
|September 20, 2024
概括
一种新的基于CRISPR的测定方法,RECOGNIZER,使用商业带快速检测人类呼吸道病毒 (HRVs). 这种用户友好的平台为改善流行病应对工作提供了巨大的潜力.
科学领域:
- 生物技术是生物技术.
- 分子诊断学 分子诊断
- 流行病的准备 流行病的准备
背景情况:
- 新兴的人类呼吸道病毒 (HRV) 流行需要敏感的,多重的,临床诊断测试.
- 目前的诊断局限性阻碍了对病毒爆发的快速反应.
研究的目的:
- 开发一种创新的,用户友好的基于CRISPR的测定方法,用于快速,多重检测多个HRV.
- 在流行病情景中解决对可访问和高效诊断的关键需求.
主要方法:
- 开发RECOGNIZER (通过大尺寸的纳米花来重建商业妊娠条),一种单CRISPR测试.
- 利用DNA纳米花来实现高探测载荷,并最大限度地减少非特异性相互作用.
- 集成多通道芯片和定制应用程序,在25分钟内进行样本到答案分析.
主要成果:
- 在1nM的目标度下,RECOGNIZER实现了超过10倍的信号噪声比.
- 对于合成SARS-CoV-2RNA的感应度为5.42 pM,而对于等离子体则为10副本μL-1.
- 在50个临床抽样样中,在区分SARS-CoV-2与其他HRV方面展示了100%的准确性.
结论:
- RECOGNIZER提供了一个多功能,可扩展和用户友好的平台,用于快速监控HRV.
- 该测试显著提高了对新出现的病毒威胁及时响应的能力.
- 这项技术在改善全球流行病准备和应对方面具有巨大的潜力.
相关概念视频
CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
The Antiviral System of Bacteria and Archaea: CRISPR
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
CRISPR/Cas9 Genome Editing
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

