在便样本中对样本度进行色度校正
Joris R Delanghe1, Jan Van Elslande1, Maaike J Godefroid1
1Labo Maenhout, Waregem, Belgium.
Clinical chemistry and laboratory medicine
|September 20, 2024
概括
使用stercobilin对便免疫化学测试 (FIT) 和便calprotectin结果进行校正,可以改善解释. 这种方法可以解释便样本的稀释,从而更准确地检测结直肠癌和肠道炎症.
科学领域:
- 临床化学 临床化学
- 胃肠病学 胃肠病学
- 生物化学 生物化学
背景情况:
- 便免疫化学测试 (FIT) 和便calprotectin是分别用于结直肠癌查和肠道炎症监测的标准.
- 分析前的问题,特别是可变的便含水量,挑战了这些测试的准确性.
- 目前,没有参考分析物存在,以标准化基于样本水分的结果.
研究的目的:
- 作为参考分析物,用于纠正便样本的分析前变异,对斯特科比林进行研究.
- 评估基于斯特科比林的校正对FIT和便calprotectin结果的解释的影响.
主要方法:
- 稳定的血代谢物斯特科比林在便提取物中使用色度计 (I指数) 进行了测试.
- 使用Atellica平台测量血清指数 (H,I,L) 和胆红素度.
- 中位数I指数被用来纠正便样本稀释.
主要成果:
- 斯特科比林度显示出高个体间的差异,允许对样本稀释进行校正.
- 纠正FIT结果增加了阳性测试,从9.3%增加到11.7%.
- 便calprotectin校正产生了3.1%更多的积极结果和7.7%更多的负面结果.
结论:
- 基于斯特科比林的校正适用于FIT和便calprotectin,除了阻塞性黄.
- 为样本稀释调整结果可以提高对常见便分析物的解释.
- 这种方法可能会导致对结直肠癌和炎症性肠病的临床决策更加精确.
相关概念视频
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