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在便样本中对样本度进行色度校正.

Joris R Delanghe1, Jan Van Elslande1, Maaike J Godefroid1

  • 1Labo Maenhout, Waregem, Belgium.

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概括
此摘要是机器生成的。

使用stercobilin对便免疫化学测试 (FIT) 和便calprotectin结果进行校正,可以改善解释. 这种方法可以解释便样本的稀释,从而更准确地检测结直肠癌和肠道炎症.

关键词:
这是一种calprotectin.便中的隐性血液.在分析前的错误.这种菌素是 stercobilin.

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科学领域:

  • 临床化学 临床化学
  • 胃肠病学 胃肠病学
  • 生物化学 生物化学

背景情况:

  • 便免疫化学测试 (FIT) 和便calprotectin是分别用于结直肠癌查和肠道炎症监测的标准.
  • 分析前的问题,特别是可变的便含水量,挑战了这些测试的准确性.
  • 目前,没有参考分析物存在,以标准化基于样本水分的结果.

研究的目的:

  • 作为参考分析物,用于纠正便样本的分析前变异,对斯特科比林进行研究.
  • 评估基于斯特科比林的校正对FIT和便calprotectin结果的解释的影响.

主要方法:

  • 稳定的血代谢物斯特科比林在便提取物中使用色度计 (I指数) 进行了测试.
  • 使用Atellica平台测量血清指数 (H,I,L) 和胆红素度.
  • 中位数I指数被用来纠正便样本稀释.

主要成果:

  • 斯特科比林度显示出高个体间的差异,允许对样本稀释进行校正.
  • 纠正FIT结果增加了阳性测试,从9.3%增加到11.7%.
  • 便calprotectin校正产生了3.1%更多的积极结果和7.7%更多的负面结果.

结论:

  • 基于斯特科比林的校正适用于FIT和便calprotectin,除了阻塞性黄.
  • 为样本稀释调整结果可以提高对常见便分析物的解释.
  • 这种方法可能会导致对结直肠癌和炎症性肠病的临床决策更加精确.