对于一氧化碳的现场生物成像,NIR-II正交光电比度纳米探针用于一氧化碳的生物成像
Lichao Guo1, Qingyuan Wang2, Feng Gao1
1School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
Small (Weinheim an der Bergstrasse, Germany)
|September 20, 2024
概括
这项研究引入了一种新的近红外II (NIR-II) 光纳米探针,用于准确的体内一氧化碳 (CO) 检测. 这一进步使CO相关疾病的精确生物成像和诊断成为可能,克服了传统方法的局限性.
科学领域:
- 生物医学工程 生物医学工程
- 化学生物学 化学生物学
- 医疗成像医学成像
背景情况:
- 一氧化碳 (CO) 是一个关键的内源信号分子,参与许多生理和病理过程.
- 在体内检测CO的传统光成像方法受到自光,光子散射和复杂生物环境中的信号不稳定性的限制.
- 准确检测二氧化碳对于了解其在疾病中的作用和开发有效的诊断工具至关重要.
研究的目的:
- 开发一种新的近红外II (NIR-II) 光纳米探针,用于对一氧化碳 (CO) 进行敏感和准确的体内比度生物成像.
- 克服传统光成像的局限性,包括背景噪音和信号波动,以可靠地检测CO.
- 建立一种新的工具,用于精确诊断CO相关疾病,如急性肝损伤,使用体内成像.
主要方法:
- 开发一个NIR-II光纳米探测器 (NP-Pd) 使用下转换纳米粒子 (DCNP).
- 采用双波长激发和辐射检测的直角比度光策略的实施.
- 在小鼠模型中验证纳米探针的性能,用于CO的体内生物成像和急性肝损伤的诊断.
主要成果:
- NP-Pd纳米探测器在808nm的CO存在下显示了吸收的响应增强,在1060nm放大了DCNP光信号.
- 使用DCNP信号在1525 nm的980 nm激发下建立了一个稳定的内部光标准,不受CO的影响.
- 在小鼠模型中实现了准确的体内比值CO生物成像和急性肝损伤的精确诊断.
结论:
- 开发的NP-Pd纳米探测器为NIR-II窗口中CO的体内比度生物成像提供了一个强大而准确的平台.
- 这种新的方法有效地减轻了与生物成像中的自流体和信号不稳定性相关的挑战.
- 纳米探针作为一种有价值的工具,可以促进CO相关病理的体内检测和诊断.
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