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干扰素兰巴达在中性粒细胞中的信号增强了Bordetella pertussis感染的发病因子

Amit Kumar¹, Da'Kuawn Johnson¹, Alicia Bukowski¹

¹Department of Microbiology and Immunology, University of Maryland School of Medicine, 685 W. Baltimore St., Baltimore, MD 21201, United States.

Journal of leukocyte biology

|September 20, 2024

概括

干扰素兰巴达阻碍了中性粒细胞细菌在 Bordetella pertussis 感染中的杀死. 在小鼠中阻断这种途径减少了细菌负荷和肺炎,揭示了治疗点.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
传染性疾病传染性疾病

背景情况:

干扰素兰巴达 (IFN-λ) 与细菌感染有关,可能会加剧肺炎.
之前的研究表明,在 Bordetella pertussis 感染的小鼠中,IFN-λ 诱导.

研究的目的:

调查干扰素兰巴受体1 (IFNLR1) 在B. pertussis感染中中性粒细胞的作用.
确定百日咳毒素如何影响中性粒细胞功能和IFN-λ信号传递.

主要方法:

使用MRP8creIFNLR1fl/fl小鼠缺乏IFNLR1,特别是在中性粒细胞上.
在野生类型和淘汰赛小鼠之间比较细菌负载,肺炎,中性粒细胞功能和细胞因子概况.
评估了IFN-λ和百日咳毒素对中性粒细胞杀死能力的影响.

主要成果:

缺乏中性细胞IFNLR1的小鼠显示细菌负载减少和肺炎.
用IFN-λ治疗的野生型中性粒细胞显示细菌杀死率降低.
百日咳毒素增强了B.百日咳在野生型小鼠中的负载,但不是在IFNLR1缺乏的小鼠中,通过增强的III型IFN信号传递进行介导.

结论:

中性细胞的IFNLR1信号传递,由百日咳毒素增强,损害了中性细胞对抗B. pertussis的功能.
针对III类型的IFN信号提供了B. pertussis感染的潜在治疗策略.

关键词:

百日咳 Bordetella 百日咳是一种疾病.干扰剂干扰剂干扰剂干扰剂干扰素兰巴达是一种干扰素.中性粒细胞中性粒细胞.百日咳毒素是一种毒素.呼吸道感染呼吸道感染

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