相关实验视频
Updated: Aug 10, 2026
11:38
A Quantitative Glycomics and Proteomics Combined Purification Strategy
Published on: March 8, 2016
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nQuant 能够实现精确的定量N-糖组合物
Yudong Guan1, Shanshan Zhao2, Chunjin Fu3
1Department of Critical Care Medicine, Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics, Shenzhen People's Hospital, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, Guangdong 518020, China.
Analytical chemistry
|September 20, 2024
概括
我们开发了nQuant,这是一个新的甘氨酸信息学工具,用于精确的N-甘氨酸量化. 该工具支持无标签和同位素标签方法,改进了从质谱学分析N-glycomics数据的分析.
科学领域:
- 生物化学 生物化学
- 格莱科米克斯 (Glycomics) 是一个有趣的药物.
- 生物信息学是一种生物信息学.
背景情况:
- N-糖基化是一种关键的翻译后修改,影响蛋白质功能.
- 异常的N-葡萄糖化与许多人类疾病有关.
- 目前的甘氨酸信息学工具缺乏全面的N-甘氨酸量化所需的精度.
研究的目的:
- 开发一种新的甘氨基信息学工具,nQuant,用于准确有效地量化N-甘氨基.
- 为了使无标签和同位素标签量化N-glycomics数据.
- 解决高精度N-甘氨酸分析现有工具的局限性.
主要方法:
- 开发nQuant软件工具,用于N-glycomics数据分析.
- 使用液体染色学-并联质谱法 (LC-MS/MS) 来获取数据.
- 在nQuant.范围内实施无标签和同位素标签量化模块.
主要成果:
- nQuant证明了N-glycans的低错误量化率.
- 从纯化糖蛋白,HEK293细胞中分析的N-甘氨酸,以及在小鼠体胆发育过程中使用无标签量化.
- 通过同位素标记量化,在治疗后的急性前兆细胞性白血病细胞中揭示了动态的N-甘氨酸变化.
结论:
- nQuant提供了一种快速而精确的方法来定量N-糖化合物.
- 该工具有助于在各种生物环境中研究N-糖基化动态.
- nQuant增强了用于疾病研究的复杂糖性成分数据分析的能力.
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