相关实验视频

Updated: Jun 12, 2025

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

脂肪-Dachsous平面极性函数需要两个不同的异性恋阴素-阴素结合相互作用

Helen Strutt¹, Dipak Meshram¹, Elizabeth Manning¹

¹School of Biosciences, University of Sheffield, Sheffield S10 2TN, UK.

|September 20, 2024

概括

脂肪和Dachsous,非典型的cadherins,在他们的C-终端重复中有一个第二个结合点. 这种双重相互作用场所在体内影响它们的稳定性和功能,可能形成类似格子的阵列.

科学领域:

细胞生物学细胞生物学
发展生物学发展生物学
生物化学生化学

背景情况:

脂肪和 Dachsous 是进化保存的非典型cadherins.
它们调节极化细胞行为,包括Drosophila的翅膀形状.
之前的研究发现了Fat和Dachsous之间的N-终端转变相互作用.

研究的目的:

为了识别和描述Fat和Dachsous之间的额外的异性恋结合点.
在体内研究这些结合位点的功能意义.
为了阐明Fat和DachsousN-终端重复的相互作用方式.

主要方法:

生物化学测试以确定C端结合部位.
在Drosophila melanogaster体内研究以评估Fat-Dachsous功能.
对N端重复相互作用的分析.

主要成果:

在Fat和Dachsous的C端卡德林重复中确定了第二个异性恋结合部位.
这个C端部位在人类Fat4和Dachsous1.1中保留着.
无论是N端和C端的结合部位都调节了Fat-Dachsous相互作用的稳定性,N端部位在体内部分可用.
证实N端重复在体内以反平行方式相互作用.
多个结合点可能会促进脂肪-Dachsous聚合成类似格子的阵列.

结论:

脂肪和Dachsous具有多个保存的异性恋结合点.
这些部位有助于脂肪-Dachsous相互作用的稳定性和调节.
这些发现表明,一个多个结合点促进有序Fat-Dachsous数组形成的模型,这对于极化细胞行为至关重要.

关键词:

CP: 细胞生物学细胞生物学 CP: 发育生物学达克索斯是什么意思达克索斯是什么意思这种植物是Drosophila.胖胖胖的胖胖的胖胖的脂肪Dachsous路径的路径.这是PCP的PCP.卡德林是个非常有趣的人.发展发展发展发展发展.平面细胞的极性是平面的.平面极性的平面极性.机翼图案机翼图案机翼图案

更多相关视频

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Published on: August 13, 2012

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

Published on: February 21, 2016

相关概念视频

Structure of Cadherins

Structure of Cadherins

The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...

Catenins

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...

Cadherins in Tissue Organization

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...

Desmosomes

Desmosomes

The term desmosome derives from the Greek words "desmo" and "soma" meaning "adhesion bodies." This structure was first observed during the late 1800s and described as small, dense nodules in the epidermis. Desmosomes are button-like structures that help form an interlinked network of intermediate filaments across the cells. These junctions are essential to hold cells together under mechanical stress and to maintain tissue integrity. Desmosomes are multi-protein...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Mechanism of Filopodia Formation

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...