通过乌比奎丁介导的SlPsbS降解调节番茄的低夜间温度耐受性
Jiazhi Lu1, Junchi Yu2, Pengkun Liu3
1The Modern Facilities Horticultural Engineering Technology Center, Shenyang Agricultural University, Shenyang 110866, China; The Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang 110866, China; Yazhouwan National Laboratory, Sanya 572024, China.
Cell reports
|September 20, 2024
概括
低夜间温度通过通过一种新的质囊 (CV) 和COP9信号体 (CSN5A) 途径降解PsbS蛋白来降低非光化学火 (NPQ). 这种机制在环境压力下调节叶绿体蛋白质的稳定性.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- PsbS蛋白对于非光化学火 (NPQ) 诱导至关重要,特别是在夜间低温 (LNT) 下.
- 在环境压力下PsbS和其他叶绿体蛋白的稳定性尚未完全理解.
- 环境因素可能会对蛋白质的稳定性产生负面影响,影响植物生理反应.
研究的目的:
- 在LNT条件下阐明控制PsbS蛋白稳定性的调节机制.
- 确定参与PsbS蛋白质降解途径的关键成分.
- 提供关于在压力下对叶绿体蛋白质稳态的见解.
主要方法:
- 研究了LNT对NPQ水平和西红中的SlPsbS蛋白质丰度的影响.
- 描述了LNT激活的叶绿体囊泡 (SlCVs) 在SlPsbS出口和降解中的作用.
- 利用遗传方法 (过度表达和沉默) 来研究SlCV和COP9信号酶子单元5A (SlCSN5A) 的参与.
- 检查了细胞质中无处不在和降解过程.
主要成果:
- LNT显著降低了番茄中的NPQ水平和SlPsbS蛋白质丰富度.
- 由LNT激活的SlCVs促进了从叶绿体中SlPsbS的出口.
- 通过与SlCV和SlCSN5A的相互作用,SlPsbS在细胞质中无处不在并降解.
- 过度表达SlCV增强了SlPsbS的降解,而沉默SlCSN5A或SlCV则延迟了LNT诱导的NPQ减少和SlPsbS的周转.
结论:
- 已经确定了一种新的,依赖于全方位素的质体蛋白质降解途径,涉及质体囊泡 (CV) 和COP9信号体子单元5A (CSN5A).
- 这一途径调节了SlPsbS蛋白循环和在LNT下减少NPQ.
- 这些发现提供了管理质细胞蛋白质稳定性的基本理解,以应对环境压力.
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