在乳牛的断奶前期确定甲原细菌相互作用
Nagaraju Indugu1, Kapil S Narayan1, Meagan L Hennessy1
1Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA, United States of America.
PloS one
|September 20, 2024
概括
乳牛的早期生命是甲素殖民的关键. 了解这些早期的相互作用可以减少甲排放,而不会影响未来的消化.
科学领域:
- 环境微生物学 环境微生物学
- 动物生物学 动物生物学
- 农业科学 农业科学
背景情况:
- 在全球范围内,动物是人类甲排放的重要来源.
- 在消化道中的甲原体产生肠道甲.
- 幼年肠道殖民和小牛中的微生物相互作用仍未得到充分研究.
研究的目的:
- 调查新生儿奶牛中从出生到断奶的甲原始社区组成.
- 为了确定新生婴儿小牛肠道中甲原体和细菌之间的相互作用的时间.
主要方法:
- 从出生到12周,每两周收集十只霍尔斯坦小牛的便样本.
- 使用16S rRNA amplicon测序对甲基原始物种多样性的分析.
- 通过定量实时PCR (RT-qPCR) 来量化甲原体.
主要成果:
- 随着时间的推移,考古社区多样性 (alpha和beta) 发生了显著的变化.
- 在早期样本 (2-6周) 中,Methanobrevibacter占主导地位,而Methanosphaera则在晚些时候 (10-12周) 增加.
- 在Methanobrevibacter和Methanosphaera之间存在反向相关性,形成与成年小鼠相似的独特细菌协会.
结论:
- 甲原始社区及其细菌相互作用是在断奶前时期建立的.
- 断奶前阶段提供了一个关键的窗口来干预甲素殖民.
- 针对生命早期的殖民化可以减少肠道甲排放,而不会影响以后的小功能.
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