功能性生衍生细胞外囊膜涂层ZIF-8含TNF-αsiRNA用于性结肠炎治疗,通过调节肠道微生物群
Chenyang Cui1, Miao Du1, Yihang Zhao1
1School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan, China.
ACS applied materials & interfaces
|September 20, 2024
概括
这项研究引入了一种新的口服治疗性结肠炎 (UC),使用生细胞外囊泡和ZIF-8纳米颗粒来提供抗瘤缩因子-α siRNA. 治疗有效地准结肠,减少炎症,恢复肠道健康.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术纳米技术
- 胃肠病学 胃肠病学
背景情况:
- 性结肠炎 (UC) 的发病包括瘤缩因子-α (TNF-α).
- 针对UC治疗的向siRNA输送在肠道微环境中面临挑战.
- 现有的治疗方法需要提高特定部位的稳定性和疗效.
研究的目的:
- 开发一种针对结肠的新型口服输送系统,用于抗TNF-α siRNA.
- 使用功能化纳米颗粒来提高性结肠炎 (UC) 的治疗疗效.
- 调查生衍生成分和siRNA输送的协同效应.
主要方法:
- 使用植物衍生生细胞外囊 (EVs) 和ZIF-8纳米粒子制造EVs@ZIF-8@siRNA纳米粒子.
- 对纳米粒子准,稳定性和siRNA负载能力的评估.
- 在UC小鼠模型中评估治疗疗效,减少炎症和肠道微生物群的调节in vivo.
主要成果:
- 生EVs表现出显著的结肠和巨细胞向,耐胃酸性.
- EVs@ZIF-8@siRNA纳米粒子有效地加载siRNA,并促进了 lysosomal 逃逸.
- 活体研究显示TNF-α在结肠组织中的显著减少,改善了肠道屏障功能,并恢复了肠道微生物群平衡.
结论:
- 开发的EVs@ZIF-8@siRNA纳米颗粒代表了UC的有希望的结肠向口服药物输送策略.
- 生EVs提供固有的抗炎性质,并增强siRNA治疗结果.
- 这种新的纳米医学方法通过调节炎症和肠道微生物群,为性结肠炎治疗提供了潜在的新途径.
相关概念视频
Drugs for Treatment of Ulcerative Colitis in IBD
129
Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
129
Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF
119
Tumor Necrosis Factor (TNF), a proinflammatory cytokine, contributes significantly to the inflammation seen in Crohn's disease. It exists as soluble TNF and membrane-bound TNF, with actions mediated through TNF receptors (TNFR). TNFR activation leads to the release of proinflammatory cytokines, T-cell activation, collagen production, and leukocyte migration, all contributing to inflammation in Crohn's disease. Anti-TNF monoclonal antibodies, namely infliximab (Remicade), adalimumab...
119
Inflammatory Bowel Disease IV: Pharmacological Management
118
Upon diagnosis, managing Inflammatory Bowel Disease (IBD) involves addressing several crucial aspects. The primary goals include resting the bowel, correcting malnutrition, and providing symptomatic relief. Resting the bowel may consist of medications to reduce inflammation and promote healing. Correcting malnutrition is essential, often requiring dietary adjustments and nutritional supplements. Symptomatic relief aims to ease pain, diarrhea, and other discomforts in IBD.
Pharmacologic...
Pharmacologic...
118
Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents
145
Crohn's disease is an inflammatory bowel disorder marked by chronic inflammation of the GI tract. Various treatment strategies for Crohn's disease are employed, such as immunomodulatory agents, glucocorticoids, and biologics or anti-TNF therapy. Azathioprine (Imuran), a commonly used immunomodulatory drug for Crohn's disease, is converted in the body to mercaptopurine, which inhibits purine biosynthesis and cell proliferation. Both are utilized in severe cases of Inflammatory Bowel...
145
Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids
98
Glucocorticoids, a class of anti-inflammatory drugs, are pivotal in treating moderate to severe Crohn's disease by inducing remission. They exhibit their anti-inflammatory action by inhibiting the production of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and chemokines like IL-8. In addition, they reduce the expression of inflammatory cell adhesion molecules and inhibit gene transcription of nitric oxide synthase, phospholipase A2, cyclooxygenase-2...
98
Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
323
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
323


