在驱逐剂适应过程中,CheB定位到极性受体阵列
Hajime Fukuoka1, Keisuke Nishitani1, Taiga Deguchi1
1Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
Science advances
|September 20, 2024
概括
大肠杆菌中的适应酶CheB甲基酶在暴露于驱逐剂时与受体阵列结合,并在适应时脱离. 这种动态局部化调节了细胞对刺激的反应.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 细胞生物学 细胞生物学
背景情况:
- 生物对刺激的适应对于生存至关重要.
- B甲基化酶是埃舍里希亚大肠杆菌信号适应的关键.
研究的目的:
- 为了研究CheB甲基化酶在大肠杆菌的适应反应中的动态.
- 量化CheB在调节受体阵列信号输出中的作用.
主要方法:
- 在刺激暴露后跟踪CheB定位到受体阵列.
- 量化CheB和细胞质CheB水平的变化.
- 在受体阵列内识别CheB结合位.
主要成果:
- 在暴露于l-异黄素后,CheB甲基酶组合到ON状态受体阵列.
- 在完成适应后,CheB从阵列中脱离.
- 结合CheB主要是对化CheA的P2域进行结合.
- 刺激的强度会影响CheB局部化持续时间和鞭毛旋转偏差.
结论:
- 切伯的细胞内动力学直接调节大肠杆菌受体阵列的信号输出.
- 这项研究量化了细菌适应的基础分子机制.
更多相关视频
09:30Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
Published on: July 13, 2017
8.1K
07:31Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons
Published on: September 7, 2017
8.1K
相关概念视频
Chemotaxis and Direction of Cell Migration
3.3K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
3.3K
Cell Polarization by Rho Proteins
2.7K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.7K
Rab Proteins
3.9K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
3.9K
G-Protein Gated Ion Channels
4.5K
GPCRs are primarily responsible for our sense of smell, taste, and vision. The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
Sensory...
4.5K
Channel Rhodopsins
2.5K
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
2.5K
GPCR Desensitization
5.9K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
5.9K
