低皮诺细胞的大脑内皮细胞主要利用膜融合来内化细胞外囊泡
Jhanvi R Jhaveri1, Purva Khare1, Paromita Paul Pinky1
1Graduate School of Pharmaceutical Sciences, Duquesne University, Pittsburgh, PA, United States.
概括
同源的细胞外囊泡 (EVs) 比异构的EVs更容易被大脑内皮细胞 (BECs) 吸收. EVs主要通过膜融合进入BEC,而不是内细胞分裂,这凸显了染料选择在吸收研究中的重要性.
科学领域:
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
- 纳米医学是一种纳米医学.
背景情况:
- 细胞外囊泡 (EVs) 是有前途的药物输送载体.
- 电子细胞通常通过内细胞路径内部化,如克拉林介导和洞穴介导的内细胞.
- 了解EV吸收机制对于优化药物输送至关重要.
研究的目的:
- 研究同型与异型相互作用对EV细胞吸收的影响.
- 确定EV内部化进入大脑内皮细胞 (BECs) 的主要途径,为难以传递的细胞提供一个模型.
- 评估标签染料化学对EV吸收研究的影响.
主要方法:
- 研究了同类和异质EVs的细胞吸收到BECs.
- 使用药理抑制剂来阻断内细胞通路.
- 采用基于R18的聚变试验来评估EV进入机制.
- 脂性染料 (PKH67) 和以酶激活染料 (素) 的比较吸收.
主要成果:
- 与异型相互作用相比,同型相互作用导致BEC中EV吸收显著增加.
- 阻断内细胞通路并没有完全抑制EV吸收到BECs.
- 电动汽车主要通过膜融合进入BEC,根据融合试验结果.
- 脂性染料标签 (PKH67) 减少了EV吸收到BEC,与酶激活染料标签不同.
结论:
- 电动细胞利用膜融合作为一种主要的内化机制,进入像BEC这样的低皮诺细胞细胞.
- 同型相互作用增强了EV向目标细胞的传递.
- 标签染料的选择至关重要,并且可以显著影响EV吸收研究的解释,特别是在具有挑战性的细胞模型中.
相关概念视频
01:26Fusion of Secretory Vesicles with the Plasma Membrane
11.0K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
11.0K
01:44Introduction to Membrane Traffic
7.0K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
7.0K
01:38Intralumenal Vesicles and Multivesicular Bodies
3.4K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.4K
01:45Vesicular Tubular Clusters
2.5K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
2.5K
01:18Vesicular Trasport: Endocytosis, Transcytosis and Exocytosis
972
Vesicular transport is a cellular process that encompasses the engulfment of particles or dissolved substances by cells. It involves endocytosis, transcytosis, and exocytosis.
Endocytosis is a cellular mechanism that involves the inward folding of the cell membrane to create vesicles that capture and transport large drug molecules. This process comprises two distinct methods: pinocytosis (often referred to as "cell drinking") and phagocytosis (often referred to as "cell...
Endocytosis is a cellular mechanism that involves the inward folding of the cell membrane to create vesicles that capture and transport large drug molecules. This process comprises two distinct methods: pinocytosis (often referred to as "cell drinking") and phagocytosis (often referred to as "cell...
972
00:50Exocytosis
6.6K
Exocytosis is a process that releases molecules outside the cell. Like other bulk transport mechanisms, exocytosis requires energy.
Exocytosis is the opposite of endocytosis, which brings molecules inside the cell. Sometimes, the released materials are signaling molecules. For example, neurons typically use exocytosis to release neurotransmitters. Cells also use exocytosis to insert proteins such as ion channels into their cell membranes, secrete proteins for use in the extracellular matrix, or...
Exocytosis is the opposite of endocytosis, which brings molecules inside the cell. Sometimes, the released materials are signaling molecules. For example, neurons typically use exocytosis to release neurotransmitters. Cells also use exocytosis to insert proteins such as ion channels into their cell membranes, secrete proteins for use in the extracellular matrix, or...
6.6K