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对一种快速表型抗微生物敏感性测试系统的比较研究,直接从阳性血培养到磁盘扩散和VITEK 2方法

Merav Strauss¹, Shereen Affan Suleiman¹, Najwa Lauz¹

¹The Microbiology Laboratory, Emek Medical Center, Yitshak Rabin Boulevard 21, Afula 1834111, Israel.

Journal of microbiological methods

|September 20, 2024

概括

该dRAST系统表现出良好的抗微生物敏感性测试 (AST) 直接从积极的血液培养表现出良好的性能. 这种快速的方法显著减少了败血症患者护理的周转时间和劳动力.

科学领域:

临床微生物学临床微生物学
传染病传染病传染病
诊断技术诊断技术诊断技术

背景情况:

败血症影响全球4900万,每年造成1100万人的死亡.
快速的抗微生物敏感性测试 (AST) 对于有效的败血症治疗至关重要.
目前的方法需要及时的结果,以获得最佳的患者结果.

研究的目的:

为了评估Quantamatrix的dRAST v2.5系统对AST的性能.
直接比较dRAST与磁盘扩散 (DD) 和VITEK 2方法.
评估dRAST在临床环境中对血液感染的有用性.

主要方法:

分析了来自临床样本和尖尖瓶的191个阳性血液培养物.
使用VITEK 2,磁盘扩散 (DD) 和dRAST进行的抗菌敏感性测试 (AST).
应用了CLSI (2021) 解释标准进行AST评估.

主要成果:

dRAST 对于 Gram 阴性和 Gram 阳性分离物表现非常好,符合 CLSI 接受标准.
对于ESBL检测和其他耐药性机制观察到高一致性.
与VITEK 2和DD相比,特定的抗微生物药物显示出差异.

结论:

该dRAST系统满足大多数抗生素的CLSI标准,提供良好的AST性能.
dRAST显著减少了结果的时间,劳动力和结果分析的主观性.
dRAST是改善细菌病患者管理和败血症治疗的宝贵工具.

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