用于封装蛋白水解纳米颗粒的抗溶剂沉:对消除味道和生物可访问性的影响
Li Liu1, Weijia Zhang1, Yuanhui Zhao1
1College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China.
International journal of biological macromolecules
|September 20, 2024
概括
这项研究开发了新的谷物蛋白质甘油纳米颗粒,用于输送蛋白水解盐 (OPH). 这些稳定,无毒的纳米粒子有效地减少了异味,并增强了OPH.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物材料工程 生物材料工程
- 营养学药物开发
背景情况:
- 蛋白水解剂 (OPH) 具有宝贵的生物活性特性,但在输送和稳定性方面面临挑战.
- 淡味和氧化限制了OPH在食品中的直接应用.
- 开发有效的交付系统对于利用OPH的全部潜力至关重要.
研究的目的:
- 创建稳定,功能性的纳米颗粒,用于输送蛋白水解盐 (OPH).
- 研究纳米颗粒封装对OPH的风味特征,稳定性和生物可用性的影响.
- 评估OPH装载纳米颗粒的安全性和有效性,用于潜在的营养药物应用.
主要方法:
- 使用抗溶剂沉来合成复合纳米颗粒使用Zin,酸 (NaCas) 和Gum Arabic.
- 在纳米粒子内嵌入OPH是由结和疏水性相互作用驱动的.
- 特性包括粒子大小,多分散度指数 (PDI),泽塔潜力和二次结构分析 (里埃变换红外光谱学).
- 进行了感官评估和化学分析,以评估异味减少.
- 使用Caco-2和HT-29细胞进行了体外消化性和细胞毒性测定.
主要成果:
- 成功制备了具有高稳定性 (PDI ≈0.2) 和负表面电荷 (≈-28 mV) 的球形纳米粒子 (191 nm).
- 热处理诱导了OPH载的形变化,从α-螺旋到随机线圈.
- 观察到 (E,E) -2,4-heptadienal,八度甘和1-octanol等非风味化合物的显著减少.
- 装有OPH的纳米颗粒显示出优异的抗氧化能力,改善了体外消化能力,以及肠道细胞系中的无毒性.
- 封装保护了OPH免受降解,并掩盖了不良风味.
结论:
- 复合纳米颗粒为蛋白水解盐 (OPH) 提供了一种高效的输送系统.
- 封装减轻了异味,增强了OPH的稳定性和生物可用性.
- 这项技术支持OPH作为粉状营养药的发展,并提高了产品的质量.
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