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基于线粒体细胞染色体b基因的Babesia vogeli种群遗传学

Ansu Kumari^1,2, Divya Agnihotri³, Anil Kumar Nehra⁴

¹Department of Veterinary Medicine, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, 125004, Haryana, India. anshushehrawat1096@gmail.com.

Scientific reports

|September 20, 2024

概括

这项研究使用细胞染色体b基因序列来表征B. vogeli种群,揭示了两个子类和低遗传多样性. 在印度和中国人口之间观察到适度的遗传差异化.

科学领域:

遗传学遗传学是一个
动物学动物学
分子生物学分子生物学

背景情况:

B. vogeli物种需要种群遗传特征,以了解其进化历史和遗传多样性.
细胞染色体b (cyt b) 基因是人口遗传研究中的一个有价值的标志物,因为它相对保守的性质和存在于线粒体DNA.

研究的目的:

使用细胞染色体b (cyt b) 基因序列对B. vogeli进行种群遗传表征.
分析B. vogeli. 的遗传关系,遗传多样性和人口结构.
调查印度和中国B. vogeli种群之间的地理结构和基因流动.

主要方法:

从GenBank获得的B. vogeli细胞染色体b (cyt b) 基因序列 (≥685 bp) 的遗传学分析.
鉴定核酸变异,同义和非同义替代,以及已识别的子类之间的氨基酸变化.
核酸和单种型多样性,F_ST和Nm值的计算,以评估遗传差异化和基因流动.
构建一个连接中位数的单双型网络,并应用中性测试和不匹配分布分析.

主要成果:

遗传学分析将B. vogeli分为一个单一单一类的两个子类 (Bv1和Bv2).
在子类之间观察到七种核酸变异,包括三种非同义替代导致氨基酸变化.
在B. vogeli种群中发现了低核酸和单元型多样性,只确定了两个单元型.
在印度和中国人群之间检测到中度的遗传差异化 (F_ST = 0.05) 和高基因流 (Nm = 4.75).
中立性测试和不匹配分布表明印度人口和B. vogeli总体数据集的种群大小是恒定的.

结论:

这项研究提供了基于细胞染色体b (cyt b) 基因的B. vogeli第一个全面的人口遗传特征.
这些发现揭示了B. vogeli种群中独特的遗传结构,印度和中国之间存在显著的基因流动.
结果表明B. vogeli的种群规模稳定,有助于我们了解其进化动态.

关键词:

B. vogeli 的意思是说.细胞染色体b基因的发生.狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗遗传多样性遗传多样性哈普洛型是指一种类型.

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