对从怀疑患有子宫内膜和淋巴结结核病的患者中采集的甲固定型氨酸嵌入式活检样本进行的分子和细菌学检测方法的评估
Negash Baye1,2, Abay Atnafu3, Selfu Girma3
1Armauer Hansen Research Institute, Addis Ababa, Ethiopia. negila83@gmail.com.
BMC infectious diseases
|September 20, 2024
概括
分子和细菌学的测试提供更高的检测率为子宫内膜结核病比传统的病原体在埃塞俄比亚. 将这些方法与组织病理学相结合,可以改善诊断并减少不适当的抗菌素使用.
科学领域:
- 妇科 妇科 妇科 妇科
- 传染性疾病 传染性疾病
- 分子诊断学 分子诊断学
背景情况:
- 子宫内膜结核 (TB) 是一个重要的妇科问题,影响生育能力和生活质量.
- 组织病理学,目前的标准,对诊断子宫内膜结核病的特异性较低.
- 关于细菌学和分子方法在埃塞俄比亚诊断子宫内膜结核病的有用性的证据有限.
研究的目的:
- 研究分子和细菌学方法对子宫内膜和淋巴结结结核病的检测率.
- 为了比较Auramine O染色的诊断性能,GeneXpert MTB/RIF测定和实时PCR与组织病理学.
- 评估这些方法在像埃塞俄比亚这样资源有限的环境中的实用性.
主要方法:
- 一项回顾性横截面研究分析了从2018年至2022年的90个甲固定嵌入式活检样本.
- 样品使用Auramine O染色,GeneXpert MTB/RIF测定和实时PCR进行了测试.
- 组织病理学作为参考标准;统计分析包括科恩的卡帕协议.
主要成果:
- 实时PCR显示了最高的检测率 (60%),其次是GeneXpert MTB/RIF (47.8%) 和Auramine O (36%).
- 实时PCR显示出更高的灵敏度 (70%),其次是GeneXpert MTB/RIF (58.6%).
- 奥拉胺O和分子方法与组织病理学一致性较低 (卡帕值=0.2).
结论:
- 分子和细菌学的方法提供更好的检测率为子宫内膜结核病相比,他的病理学.
- 仅仅依靠组织病理学来做出治疗决策可能会导致不良结果.
- 建议将分子和细菌学测试与组织病理学进行整合,以尽量减少在资源有限的环境中不适当使用抗菌剂.
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