马尔迪-托夫质谱的应用用于识别诺卡迪亚物种
Ya Liu1,2, Si-Ying Wu1,2, Jin Deng1,2
1Division of Clinical Microbiology, Department of Laboratory Medicine, West China Hospital Sichuan University, No. 37 Guo Xue Xiang, Chengdu, 610041, Sichuan, P.R. China.
BMC microbiology
|September 20, 2024
概括
准确识别诺卡迪亚物种对于治疗无心病至关重要. 使用更新的数据库和提取方法的飞行质谱法 (MALDI-TOF MS) 的矩阵辅助激光脱/离子化时间显著提高了Nocardia识别的准确性.
科学领域:
- 临床微生物学 临床微生物学
- 传染病 传染病 传染病
- 质谱测量质量谱测量
背景情况:
- 无心脏病是一种严重的感染,患病率和死亡率很高.
- 准确和快速识别诺卡迪亚物种对于有效治疗至关重要.
- 飞行质谱学 (MALDI-TOF MS) 的矩阵辅助激光脱吸/电离时间为快速识别提供了一个潜在的解决方案.
研究的目的:
- 为了评估MALDI-TOF MS的性能,使用不同的数据库版本来识别Nocardia物种.
- 为了比较 Bruker MALDI Biotyper 3.1 和 MBT Compass 4.1 与更新的数据库的准确性.
- 评估一种新的提取方法对诺卡迪亚鉴定的影响.
主要方法:
- 用16S rRNA/hsp65/gyrB/secA1/rpoB基因测序作为黄金标准识别了76个诺卡迪亚临床分离物.
- 用BDAL数据库对Bruker MALDI生物型号3.1 (V09.0.0.0_8468) 和MBT指南针4.1 (V11.0.0.0_10833) 的评估.
- 应用一种新的,简化的提取方法来准备样品.
主要成果:
- 基因测序在76个分离物中确定了12种诺卡迪亚物种.
- 马尔迪生物型号3.1实现了100%的属级准确性和84.2%的物种级准确性.
- 随着更新的数据库,MBT Compass 4.1 提高了物种识别的准确性,达到 98.7%.
- 更新的数据库显著提高了物种识别率 (P=0.001).
- 新的提取方法提高了物种级别识别率 (P=0.016).
结论:
- MALDI-TOF MS对于诺卡迪亚的识别是准确的,性能因数据库版本而异.
- 与旧版本相比,带有更新数据库的MBT Compass 4.1显著提高了物种识别准确性.
- 简化提取方法可以提高识别结果,特别是对于拥有旧数据库的实验室.
- 通过优化的MALDI-TOF MS协议,可以实现准确的诺卡迪亚鉴定.
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