相关实验视频

Updated: Jun 30, 2026

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

微型化蛋白质分析允许在个体循环瘤细胞中进行有针对性的信号通路分析,以改善个性化治疗

Mahdi Rivandi^1,2, André Franken^1,2, Liwen Yang^1,2

¹Department of Obstetrics and Gynecology, University Hospital and Medical Faculty of Heinrich Heine University Duesseldorf, Duesseldorf, Germany.

Journal of translational medicine

|September 20, 2024

概括

ZeptoCTC的工作流使单细胞蛋白质分析成为可能,揭示了癌症患者的关键蛋白质激活差异,以进行个性化治疗. 这种方法促进了癌症研究,提高了治疗精度.

科学领域:

生物化学生物化学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

像循环瘤细胞 (CTC) 这样的单个细胞的基因组分析错过了蛋白质的改变,限制了治疗的有效性.
开发了一种新的工作流程,ZeptoCTC,用于单细胞蛋白质分析.
ZeptoCTC将单细胞隔离与反相蛋白阵列 (RPPA) 集成在一起,以进行全面的蛋白质评估.

研究的目的:

开发和验证ZeptoCTC工作流程,用于分析单个细胞中的蛋白质表达和激活.
为了证明ZeptoCTC在癌症研究和个性化医学中的实用性.

主要方法:

单个细胞被标记和隔离.
细胞溶解物被打印到ZeptoChip上,使用微操纵器.
光免疫测试RPPA用于蛋白质检测,通过Image J软件进行信号量化.

主要成果:

ZeptoCTC准确地测量了不同细胞系中的EpCAM蛋白表达.
它检测到治疗细胞中的pAkt/Akt比率增加了2倍,与西方斑点结果一致.
与WBC相比,ZeptoCTC揭示了转移性乳腺癌患者的CTC中pAkt和pErk激活的显著差异,以及基于Akt1基因型的差异化CTC.

结论:

ZeptoCTC 工作流是单细胞癌症研究和个性化医学的宝贵工具.
它能够详细分析向信号通路中的蛋白质激活,从而提高对瘤反应和治疗疗效的理解.
这种方法推进了临床癌症研究,提高了治疗的精度和有效性,在干细胞,神经病理学和血液细胞研究中具有潜在的应用.

关键词:

乳腺癌乳腺癌乳腺癌循环的瘤细胞循环.个性化医疗是个性化的医疗.蛋白质分析蛋白质分析单细胞蛋白质组学单细胞蛋白质组学

更多相关视频

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Published on: May 14, 2019

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

相关概念视频

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...

Combination Therapies and Personalized Medicine

Combination Therapies and Personalized Medicine

Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...