乌斯蒂拉戈公司的曼诺西利利里醇脂质料批量生产可能会从水友性碳源中制DSM 4500
André D Valkenburg1, George M Teke1, Robert W M Pott1
1Department of Chemical Engineering, Stellenbosch University, Stellenbosch, 7602, South Africa.
Bioprocess and biosystems engineering
|September 21, 2024
概括
研究人员探索了使用水友糖而不是昂贵的油来生产曼诺西利利利醇脂 (MELs). 这种方法实现了0.87 g/L MEL-A,微量营养素提高了产量24.1%,提高了化品和制药应用的经济可行性.
科学领域:
- 生物技术是生物技术.
- 生物化学 生物化学
- 微生物发酵 微生物发酵
背景情况:
- 曼诺赛利利醇脂 (MELs) 是一种有价值的生物表面活性剂,在化品和药品中具有应用,特别是用于治疗皮肤.
- 传统的MEL生产使用昂贵的植物油,使下游加工复杂化,并阻碍经济可行性.
- 开发具有成本效益和高效的MEL生产方法对于其更广泛的应用至关重要.
研究的目的:
- 为了研究使用Ustilago maydis.使用水友性碳来源的MEL-A的生产.
- 通过食批量策略优化MEL-A产量并评估微量营养素的影响.
主要方法:
- 使用葡萄糖作为唯一的碳来源种植Ustilago maydis DSM 4500.
- 实施料批发发酵策略,以最大限度地提高MEL-A度.
- 评估微量营养素补充剂,特别是硫酸 (MnSO4) 对MEL-A生产的影响.
主要成果:
- 仅使用葡萄糖实现了0.87g/L的最大MEL-A度.
- 补充微量营养素,如MnSO4,导致MEL-A标位增加24.1%.
- 结果表明,在微量营养素的存在下形成的某些代谢物有利于MEL的产生.
结论:
- MEL-A可以有效地从像葡萄糖这样的含水性碳来源中生产,为植物油提供了潜在的更经济的替代品.
- 料批发发酵和微量营养素补充是提高MEL-A产量的可行策略.
- 对特定代谢物作用的进一步研究可能会导致化品和制药行业更高效的MEL生产过程.
相关概念视频
01:22Bioreactor Controls-III
70
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
70
01:27Upstream Processing
102
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
102
01:25Production of Organic Acids
111
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
111
01:27Production of Antibiotics
281
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
281
01:30Production of Pharmaceuticals
99
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
99