用高辐射UV-A源对细菌和RNA病毒进行无活化
Karina Spunde1, Zhanna Rudevica1, Ksenija Korotkaja1
1Latvian Biomedical Research and Study Centre, Ratsupites Str. 1, Riga, 1067, Latvia.
概括
紫外线-A LED消毒有效地使细菌和包裹病毒失活. 高辐射紫外线-A显示出对无活性化不那么敏感的非包裹病毒的承诺,如MS2菌体.
科学领域:
- 微生物学 微生物学
- 摄影化学的使用.
- 环境科学 环境科学
背景情况:
- 使用灯的紫外线 (UV) 消毒正在被环保的 LED 替代品所取代.
- 紫外线-A消毒的有效性取决于病原体类型,需要优化时间/剂量关系.
- 紫外线A的病原体无活化涉及反应性氧物种 (ROS) 和直接的核酸损伤.
研究的目的:
- 为了评估10WUV-ALED (365-375nm) 对细菌和病毒的消毒效果.
- 为了确定特定病原体的UV-A无活化的时间/剂量关系.
- 探索UV-A无活化的机制,包括ROS的产生和直接的RNA损伤.
主要方法:
- 对大肠杆菌 (E. coli),MS2菌体和塞姆利基森林病毒 (SFV) 进行了UV-A无活化测试.
- 高辐射 (大约. 0.46 W/cm2) 的UV-A LED被用于消毒实验.
- 在被辐射的大肠杆菌中测量了ROS的产生,在RNA辐射后评估了病毒基因表达.
主要成果:
- 对大肠杆菌和SFV的4-log10降低剂量分别为268 J/cm2和241 J/cm2.
- 在30分钟内,MS2菌的2.5-log10降低需要679 J/cm2,这表明非包裹病毒的显著无活化.
- ROS的产生与大肠杆菌无活化相关,并直接对病毒RNA减少基因表达的UV-A损伤.
结论:
- 高辐射UV-A LED消毒对细菌和包裹病毒是有效的.
- 紫外线A显示出具有重要潜力,可以使抗紫外线A的非包裹病毒失活.
- 对病毒RNA的直接损伤是高辐射UV-ALED介导病毒失活的关键机制.
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