培养和其他直接检测方法来诊断人类颗粒细胞性厌血症
Maria E Aguero-Rosenfeld1,2, Lois Zentmaier2, Dionysios Liveris3
1Department of Pathology, Microbiology and Immunology and Department of Medicine, New York Medical College, Valhalla, NY, US.
American journal of clinical pathology
|September 21, 2024
概括
在直接的实验室测试中,培养表现为检测Anaplasma phagocytophilum (人类颗粒细胞性亚纳等离子症) 的最高产量,尽管它可能不适用于所有医院. PCR和血液涂抹显微镜的敏感度较低.
科学领域:
- 医学微生物学 医学微生物学
- 传染性疾病 传染性疾病
- 诊断 实验室科学 诊断 实验室科学
背景情况:
- 人类颗粒细胞性厌血症 (HGA) 是一种由引起的传播疾病,由Anaplasma phagocytophilum引起.
- 准确和及时的实验室诊断对于有效的患者管理至关重要.
- 直接检测方法对于早期识别Anaplasma phagocytophilum至关重要.
研究的目的:
- 为了评估三个直接实验室测试的诊断性能,针对Anaplasma phagocytophilum.
- 为了比较血显微镜,PCR和细胞培养用于HGA检测的灵敏度.
- 评估这些方法在临床环境中的有用性.
主要方法:
- 来自疑似HGA患者的血液样本使用直接显微镜检查包括,PCR和HL-60细胞系培养进行了测试.
- 使用间接免疫光检测血清学证实了病例,如果只有一个直接测试是阳性的.
- 55名患者的小组接受了所有三种直接测试方法.
主要成果:
- 在90.9%的病例中,培养物检测到Anaplasma phagocytophilum,其次是PCR (81.8%) 和血液涂抹显微镜 (63.6%).
- 在三种直接方法中,文化产生了最高的阳性率 (P = .002).
- 大多数阳性培养物 (79.3%) 在化后一周内被发现.
结论:
- 细胞培养证明了直接检测Anaplasma phagocytophilum的最高灵敏度.
- 虽然有效,但Anaplasma fagocytophilum培养可能会给常规医院实验室带来实施挑战.
- 进一步研究优化HGA的直接诊断方法是有必要的.
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