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减少 (V) 和微生物燃料电池中电活性生物膜的性能与Shewanella putrefaciens

Lili Wang¹, Yue Zhou¹, Qi Min¹

¹Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, College of Resources and Environment, Anhui Agricultural University, Hefei, 230036, China.

Journal of environmental management

|September 21, 2024

概括

微生物燃料电池 (MFC) 中的五价 (V(V)) 暴露抑制了电活性生物膜 (EAB) 的电子转移和电化学性能. 然而,V(V) 生物还原发生,细胞外聚合物质增加,并观察到上调的V(V) 还原基因.

科学领域:

环境微生物学环境微生物学
电化学电化学电化学
生物修复是一种生物修复.

背景情况:

微生物燃料电池 (MFC) 中的电子转移对于电化学活动至关重要.
五价瓦纳 (V(V)) 生物还原及其对MFC中电活性生物膜 (EAB) 的影响尚不清楚.
污染给环境带来了挑战,需要有效的补救策略.

研究的目的:

调查不同V (V) 度对MFC中的EAB性能的影响.
阐明EABs对V (V) 暴露的生物还原机制和微生物反应.
评估MFCs在处理被污染的废水方面的潜力.

主要方法:

在梯度V (V) 度 (0-100毫克/升) 下在MFC中培养带有Shewanella putrefaciens的EAB.
电化学测量包括输出电压,功率密度,极化,循环电量测量 (CV) 和电化学阻抗光谱 (EIS).
对细胞外聚合物 (EPS) 组成,通过qPCR的基因表达 (narG,nirK,gor) 和关键电子转移指标 (利黄素,cytochrome c,NADH,ETSA) 的分析.

主要成果:

增加的V (V) 度导致MFC电压,功率密度和电化学性能下降.
V(V) 作为电子受体,并被EABs降低,最佳产量在25 mg/L V(V).
EPS 含量 (蛋白质,多糖,湿性物质) 和 V 降低基因表达随着 V 降低基因表达增加,而电子转移指标显著下降.

结论:

V(V) 暴露会通过阻碍电子转移,对MFC中的EAB电化学活性产生负面影响.
EABs可以生物降解V(V),这表明修复的潜力.
了解微生物对V (V) 的反应是优化污染废水处理中MFC的关键.

关键词:

生物减排可以减少.电活生物膜是一种电活生物膜.电子转移是指电子的转移.细胞外聚合物质是细胞外的聚合物质.五价瓦纳 (V(V)) 是一种五价瓦纳.

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