一种广泛的LC-MS/MS方法,用于对临床和尸检血液样本中的100种分析物的查和定量
Dimitra Florou1, Mathew Di Rago2, Amvrosios Orfanidis3
1Department of Forensic Medicine and Toxicology, Faculty of Medicine, School of Health Sciences, University of Ioannina, University Campus, Ioannina 45500, Greece.
概括
一种新的液体染色体质谱法准确检测全血中的100多种物质,对于法医毒理学查至关重要. 这种经过验证的方法提高了滥用药物和新型精神活性物质的检测.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 液体染色学与质谱学 (LC-MS) 结合,由于其适应性和效率,在法医毒理学中被广泛使用.
- 对广泛的物质进行准确和同时检测对于法医调查至关重要.
研究的目的:
- 开发和验证一种全面的方法,用于在人体全血中同时检测100多种分析物.
- 建立一种可靠的查工具,用于滥用药物,处方药,新兴精神活性物质和其他毒素.
主要方法:
- 采用完全验证的液体-液体提取 (LLE) 方法进行样本制备.
- 使用LC-MS/MS进行的多重反应监测 (MRM) 分析被用于高灵敏度和特异性.
- 该方法被优化为从200μL血液中提取基本和中性物质.
主要成果:
- 该方法成功检测了100多种分析物,包括各种药物类和NPS,具有高灵敏度 (LOD/LOQ:0.01-5 / 0.05-20 ng/mL).
- 实现了优异的线性 (R2 = 0.9811-0.9995),可接受的精度 (3-15%日间,7-18%日内) 和准确性.
- 证实了最小的矩阵效应 (±25%) 和高分析剂稳定性 (>85%),没有观察到转移.
结论:
- 开发的LC-MS/MS方法为例行毒理学分析提供了一个敏感,特定和强大的工具.
- 这种经过验证的方法适用于临床和解剖病例中的生物样本查,有助于法医调查.
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