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对比的转录基因和代谢基因分析揭示了Dioscorea composita中甲基斯蒙酸诱导的类固醇氨酸合成的关键调节基因
Shangjie Yu1, Jiani Zhang1, Yinxing Cao1
1Institute of Biomass Engineering, South China Agricultural University, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass, Guangzhou 510642, PR China.
International journal of biological macromolecules
|September 22, 2024
概括
甲基雅斯蒙酸盐 (MeJA) 治疗可以增强 Dioscorea composita 中的 diosgenin. 这项研究揭示了MeJA.
科学领域:
- 植物生物化学 植物生物化学
- 分子生物学分子生物学
- 代谢学 代谢学 代谢学
背景情况:
- 复合神 (D. composita) 产生具有心血管益处的类固醇素.
- 对植物激素诱导的类固醇沙素生物合成调节的理解有限.
- 甲基雅斯莫纳酸 (MeJA) 是一种已知影响植物二次代谢的植物激素.
研究的目的:
- 在MeJA治疗下研究D.composita中类固醇氨酸生物合成的调节网络.
- 分析与迪奥斯基宁和类固醇沙宁相关的转录和代谢变化.
- 为了确定参与MeJA介导的类固醇氨酸生产的关键转录因子.
主要方法:
- 用MeJA治疗的D. composita的组合转录组和代谢组分析.
- 对UDP-glycosyltransferases和Cytochrome P450单氧基因酶的基因表达分析.
- 转录因子 (TFs) 与类固醇素和结构基因的相关性分析.
- 对DcWRKY11进行基因沉默,以评估其调节作用.
主要成果:
- MeJA显著增加了迪奥斯基宁的积累,但对不同的类固醇沙因显示出不同的模式.
- 参与类固醇氨酸合成的关键基因 (UGTs,CYPs) 的表达被差异调节 (上/下).
- DcWRKY11被确定为一种积极的调节剂,其沉默降低了类固醇氨酸积累.
结论:
- 在D.composita中,MeJA诱导了复杂的调节反应,用于类固醇氨酸生物合成.
- DcWRKY11在积极调节类固醇氨酸合成方面发挥着至关重要的作用.
- 这项研究提供了关于D. composita.中类固醇沙因的MeJA介导调节网络的见解.
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