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在CRISPR/Cas9的基础上对NF-YC4促进体进行编辑,可以获得高蛋白大米和大豆

Lei Wang^1,2, Seth O'Conner¹, Rezwan Tanvir¹

¹Department of Biological Sciences, Mississippi State University, Mississippi State, MS, 39762, USA.

The New phytologist

|September 22, 2024

概括

这项研究使用基因组编辑来通过去除抑制剂结合部位来增加基因表达. 这种新的方法提高了蛋白质含量,并减少了水果和大豆等作物中的碳水化合物.

科学领域:

农业生物技术农业生物技术
分子生物学分子生物学
植物科学植物科学

背景情况:

基因组编辑提供了无转基因作物改进,但大多数应用涉及基因淘汰.
增强内源基因表达是一种特征发展的替代策略.

研究的目的:

开创一种新的基因组编辑策略,通过删除转录抑制剂结合动机来增强基因表达.
通过去除大米和大豆中的RAV和WRKY抑制剂结合部位来增加NF-YC4的表达.

主要方法:

在大米 (Oryza sativa) 和大豆 (Glycine max) 的NF-YC4促进体中确定了保留的抑制剂结合基因.
利用CRISPR/Cas9技术精确删除这些在非编码促进器区域中识别的动机.
评估了编辑植物中抑制剂结合,NF-YC4基因表达,蛋白质含量和碳水化合物水平的变化.

主要成果:

删除抑制剂结合基因导致抑制剂结合减少,并显著增加NF-YC4表达.
经过编辑的米植物显示,叶子蛋白增加了68%,种子蛋白增加了17%.
编辑的大豆植物表现出叶子蛋白质增加了25%,种子蛋白质增加了11%,碳水化合物水平降低.

结论:

在非编码序列中通过有针对性的基因组删除来增强基因表达的成功策略.
这种方法为改善作物营养质量和农业生产率提供了一个强大的工具,没有转基因.
为未来的作物改进工作提供蓝图,重点是内源基因表达调制.

关键词:

这就是CRISPR/Cas9的作用.在 NF-YC4 促销者.蛋白质蛋白质是蛋白质蛋白质的组成部分.米米饭米饭米饭米饭.豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆

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