[来自内菌的生物活性二次代谢产物Aspergillus sp. CCH-1E 来自Catharanthus roseus] 这种植物的
Ya Wang1, Yu-Wu Chen2, Bo Liu3
1School of Life Science and Engineering, Lanhzou University of Technology Lanzhou 730050, China.
概括
内生菌的真菌阿斯伯吉勒斯种类 (Aspergillus sp. CCH-1E产生了12种生物活性二次代谢物,包括两种新化合物. 谢瓦龙B (化合物7) 显示出对肺癌和宫癌细胞系具有显著的抗癌活性.
科学领域:
- 自然产品化学 自然产品化学
- 药用化学 医学化学
- 菌类学 菌类学是指菌类学.
背景情况:
- 内部植物性真菌,如阿斯伯吉勒斯种类 (Aspergillus sp. 位于Catharanthus roseus等植物中的CCH-1E是一种丰富的新生物活性二次代谢物的来源.
- 研究这些代谢物可以导致发现新的治疗剂.
研究的目的:
- 隔离和识别来自内菌的生物活性二次代谢物Aspergillus sp. 在CCH-1E.
- 评估这些分离化合物的抗癌潜力与人类癌症细胞系.
主要方法:
- 使用染色学技术分离二次代谢物,包括正常相,逆相染色学和高性能液态染色学 (HPLC).
- 使用光谱方法进行结构阐明,例如UV,IR,NMR和HR-ESI-MS.
- 使用甲基thiazolyldiphenyl-tetrazolium化物 (MTT) 测定对抗A549,NCI-H1650和HeLa癌细胞系的抗癌活性评估.
主要成果:
- 分离和鉴定了12种化合物,其中包括两种新型化合物 (chermesinone H和I).
- 化合物7 (切瓦龙B) 对所有测试的癌细胞系表现出强大的生长抑制,IC50值在1.22至2.43μmol·L-1.1之间.
- 1-6类化合物表现出适度的抗癌活性,IC50值在16.24至35.28μmol·L-1.1之间.
结论:
- 这种内性真菌是Aspergillus sp. CCH-1E产生各种各样的生物活性二次代谢产物.
- 谢瓦龙B是作为抗癌剂进一步开发的有希望的候选物,因为它具有显著的细胞毒性作用.
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