相关实验视频

Updated: Jun 12, 2025

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

12.6K

新生蛋白质组的光可激活,细胞类型特定标记.

H T Evans1, T Ko2, M M Oliveira1

  • 1Center for Neural Science, New York University, New York, New York 10003, United States.

ACS chemical neuroscience
|September 23, 2024
PubMed
概括

研究人员开发了Opto-ANL,这是一种可感应光的方法,可以在特定细胞类型中精确标记新合成的蛋白质. 这种技术为研究神经科学中的蛋白质合成和其他复杂的生物过程提供了更好的时间控制和效率.

关键词:
特定细胞类型的特定细胞类型.在 de novo 蛋白质组中具有光诱导性的在mRNA的翻译过程中,mRNA的翻译摄影中的照片摄影药理学 摄影药理学

更多相关视频

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.4K
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

6.1K

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.6K