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相关概念视频

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
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淋巴细胞子集检测的协议允许参考间隔在流细胞计系统之间转移:使用既定参考间隔进行直接验证.

Mei Liu1, Sihua Yu1,2,3, Siyao Li1

  • 1National Clinical Research Center for Laboratory Medicine, The First Hospital of China Medical University, Shenyang, China.

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淋巴细胞子集的参考间隔可以在不同的流细胞计系统之间转移,简化了临床实验室诊断. 这项研究表明,成功地在多个平台上转移,不包括NK细胞,以提高准确性.

关键词:
流动细胞计量是流动细胞计量的方法.淋巴细胞子集 淋巴细胞子集参考时间间隔的参考时间间隔.转移的转移方式

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科学领域:

  • 临床免疫学临床免疫学
  • 流细胞计技术技术 流细胞计技术
  • 实验室医学 实验室医学

背景情况:

  • 流细胞计 (FCM) 在临床环境中对淋巴细胞子集检测至关重要.
  • 单平台FCM系统的普及需要标准化的参考间隔 (RI).
  • 在FCM系统之间传输RI为实验室提供了一个实用的解决方案.

研究的目的:

  • 为了研究淋巴细胞子集参考间隔 (RIs) 在不同单平台流细胞计系统中的可转移性.
  • 评估确定和转移淋巴细胞子集的RI的可行性.
  • 在NovoCyte,BriCyteE6,DxFLEX和FACSCantoII平台上进行RI比较.

主要方法:

  • 在四个FCM平台进行了对对方法比较:NovoCyte,BriCyteE6,DxFLEX和FACSCantoII.
  • 评估了已确定的淋巴细胞子集RI的可转移性.
  • 从FACSCantoII转移RI到BriCyteE6和DxFLEX系统 (不包括NK细胞),并通过计算偏差 (CV) 来验证它们.

主要成果:

  • 在四个评估的FCM系统中,淋巴细胞子集检测结果是可比的.
  • 在测试的FCM系统之间转移淋巴细胞子集的RI是可行的.
  • 转移的RI显示,与已确定的RI相比,大多数参数的变化系数 (CV) 低于20%,但不包括NK细胞.

结论:

  • 淋巴细胞子集的参考间隔可以在不同的流细胞计系统之间成功转移.
  • 在NovoCyte,BriCyteE6,DxFLEX和FACSCantoII平台上已经证明了可转移性.
  • NK 细胞是一个例外,由于不同的定义策略,RI 不能转让.