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与从生肉和加工肉制品中分离出来的葡萄球菌相关的抗菌素耐药性和公共卫生风险
Mohammad Anas1, Showkat Ahmad Lone2, Abdul Malik1
1Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, India.
Foodborne pathogens and disease
|September 23, 2024
概括
多种耐药性葡萄球菌菌株在肉制品中普遍存在,构成公共卫生风险. 这项研究强调了食品行业需要严格的卫生和抗生素管理.
科学领域:
- 食品微生物学 食品微生物学
- 抗微生物耐药性 抗微生物耐药性
- 细菌遗传学 细菌遗传学
背景情况:
- 葡萄球菌物种是食品中常见的污染物.
- 食物传播细菌的抗菌素耐药性 (AMR) 是一个日益严重的公共卫生问题.
- 了解食物分离物的耐药性模式和基因对于风险评估至关重要.
研究的目的:
- 评估从肉类和肉制品中分离的Staphylococcus中发生的情况,抗生素耐药性和β-乳糖酶的产生.
- 在这些隔离物中识别抗菌素耐药性 (AMR) 基因.
- 评估MDR葡萄球菌在食品链中的公共卫生影响.
主要方法:
- 从街头烤肉和生牛肉中分离和识别葡萄球菌.
- 使用beta-lactam和非beta-lactam抗生素的小组进行抗生素敏感性测试.
- 对β-乳糖酶和扩展谱β-乳糖酶 (ESBL) 生产的表型检测.
- 关键的AMR基因的分子检测 (例如,mecA,Sul1,qnr).
主要成果:
- 葡萄球菌的高发病率 (75%在烤肉中,50%在生肉中).
- 对β-乳糖胺 (82%) 和非β-乳糖胺,如纳利迪克酸 (80%) 和硫法迪亚 (76%) 显著耐药.
- 82%的分离物是多药耐药 (MDR),其中48%产生β-乳糖酶,24%产生ESBLs.
- mecA (29.2%),Sul1 (25%) 和qnr基因 (20.8%) 是检测到的最常见的AMR基因.
结论:
- 在生肉和街头肉制品中,MDR菌株广泛存在.
- 这些发现表明,与受污染的肉相关的潜在公共卫生风险.
- 建议包括加强食品链卫生监测和谨慎使用抗生素.
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