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Updated: Jun 12, 2025

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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在光遗传学定位有机细胞的in situ冷相对应光和电子显微镜的精度

Vikas A Tillu1, Gregory M I Redpath2, James Rae1

  • 1The University of Queensland, Institute for Molecular Bioscience, Brisbane, Queensland 4072, Australia.

Journal of cell science
|September 23, 2024
PubMed
概括

这项研究引入了一种新的低温相关光和电子显微镜 (cryo-CLEM) 工作流程,用于快速的in situ低温电子断层扫描 (cryo-ET) 分析细胞结构. 这种方法可以更快地对有机体进行成像,而无需冷聚焦的离子束削.

关键词:
在现场冷ET.结冷-CLEMEM 的情况.内分泌体内分泌体内分泌体内分泌体内分泌体内分泌体内分泌体内分泌体内分泌体视觉遗传学 视觉遗传学

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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