在奶酪样本中分子识别与黄金葡萄球菌相关的肠毒素基因
Zahira Abdljabbar Al-Zuhairi1, Esraa Taher Muslim1, Orooba Meteab Faja1
1Collage of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq.
Open veterinary journal
|September 23, 2024
概括
病原性细菌,如金黄色葡萄球菌经常在奶酪中发现,携带肠毒素基因. 这项研究强调了乳制品中由于这些细菌污染物而存在的重大食品安全风险.
科学领域:
- 食品微生物学 食品微生物学
- 食品安全 食品安全
- 细菌病原体的产生
背景情况:
- 乳制品营养丰富,但容易受到病原性细菌污染.
- 乳制品中的细菌生长对消费者健康和经济稳定构成风险.
- 黄金葡萄球菌 (Staphylococcus aureus) 是食品安全问题中的一个重要病原体.
研究的目的:
- 在奶酪样本中检测金黄色葡萄球菌肠毒素基因.
- 评估来自奶酪的金黄色细菌中特定毒性基因 (icaA,海,hla,fnbA) 的流行率.
- 评估乳制品中S. aureus污染对食品安全的影响.
主要方法:
- 收集了35个奶酪样本进行分析.
- 采用常规培养来识别细菌.
- 使用聚合酶链反应 (PCR) 检测肠毒素基因 (icaA,海,hla,fnbA).
主要成果:
- 在奶酪样本中发现金黄色葡萄球菌.
- 肠毒素基因的高患病率: icaA (88.5%),海 (77%),hla (54%),fnbA (34%). 在这些基因中,
- 测序显示高相似性 (高达98.5%) 与全球S. aureus分离物.
结论:
- 这项研究证实,来自奶酪的金黄色菌中存在高水平的肠毒素基因.
- 这表明,乳制品消费者对食品安全存在重大担忧.
- 需要采取有效的控制措施,以减轻乳制品中S. aureus相关的风险.
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