相关实验视频
Updated: Jun 12, 2025

12:21
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
Published on: November 30, 2013
14.9K
微RNA-29b通过诱导线粒体功能障碍,在细胞损伤和淋巴细胞疾病中起着至关重要的作用
Jiafeng Liu1,2, Yabing Xiong1,2, Hongyan Mo3
1State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease, Guangdong Provincial Clinical Research Center for Kidney Disease, Guangdong Provincial Key Laboratory of Nephrology, Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
International journal of biological sciences
|September 23, 2024
概括
糖尿病病 (DKD) 患者的微RNA-29b (miR-29b) 含量升高,通过向PGC-1α,恶化细胞损伤和功能,并损害线粒体健康. miR-29b显示出作为DKD早期诊断生物标志物的潜力.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 糖尿病病 (DKD) 是末期病 (ESRD) 的主要原因之一.
- 足细胞损伤和线粒体功能障碍是DKD进展的关键驱动因素.
- 微RNAs (miRNAs) 涉及到病的发生.
研究的目的:
- 调查miR-29b在DKD病变发生中的作用.
- 确定miR-29b作为podocyte损伤和DKD的潜在生物标志物.
- 为了阐明DKD中miR-29b的分子机制.
主要方法:
- 在126名DKD患者 (I和II阶段) 的尿液分析.
- 在体外研究中使用细胞培养来评估miR-29b对细胞和PGC-1α的影响.
- 使用ADR脏病和db/db小鼠模型的体内研究,包括podocyte特定的miR-29b淘汰赛小鼠.
主要成果:
- 在DKD患者的尿液中,miR-29b水平升高,与功能和 podocyte 稳定性负相关.
- miR-29b直接准了PGC-1α,这是线粒体生物发生的调节者.
- 在体外和体内,miR-29b促进了细胞损伤和DKD进展,而其抑制或淘汰减轻了这些影响.
- 在小鼠模型中,miR-29b抑制可保持功能并减少细胞损伤.
结论:
- miR-29b通过准PGC-1α而加剧DKD,导致线粒体功能障碍和细胞损伤.
- miR-29b是一种有前途的尿路生物标志物,可用于早期检测细胞损伤和DKD.
- 向miR-29b可能为DKD提供治疗策略.
相关概念视频
The Inner Mitochondrial Membrane
3.3K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria. In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.3K
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Mitochondrial Membranes
9.2K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
9.2K
Translocation of Proteins into the Mitochondria
3.1K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.1K
Mitochondria
11.4K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
11.4K

