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在限制部位的TLR7同义变体rs864058在Covid 19中被发现巴基斯坦患者

Beenish Khalid¹, Sadia Farukh², Ashokh Kumar³

¹Department of Biochemistry, Hamdard University Karachi, Pakistan.

American journal of blood research

|September 23, 2024

概括

这项研究确定了一种新的收费类受体7 (TLR7) 基因变体 (rs864058 G/A),与巴基斯坦的COVID-19严重程度有关. 男人显示严重感染和死亡的风险增加,突出了潜在的免疫反应的基于性别的差异.

科学领域:

免疫遗传学免疫遗传学
病毒学病毒学
人口遗传学人口遗传学

背景情况:

收费类受体7 (TLR7) 对于对抗RNA病毒的免疫反应至关重要,例如SARS-CoV-2.
之前的研究发现了TLR7变种与全球COVID-19严重程度相关,但巴基斯坦人口数据缺乏.
这项研究调查了巴基斯坦COVID-19患者的TLR7基因型,以了解它们在疾病严重性中的作用.

研究的目的:

为了确定巴基斯坦人口中TLR7基因的基因型.
调查TLR7变种与COVID-19严重程度之间的关联.
为了确定TLR7基因型分布和疾病结果的潜在基于性别的差异.

主要方法:

进行了一项涉及151名COVID-19患者 (18-80岁) 的横截面研究.
从血液样本中提取DNA,然后进行聚合酶链反应 (PCR) 和桑格测序以识别TLR7变种.
使用SPSS和Chi-Square测试进行统计分析,P值<0.05被认为是显著的.

主要成果:

在59个测序样本中确定了TLR7基因变异rs864058 (G/A和G/G基因型).
G/G基因型占主导地位 (83%),而G/A在17%的患者中被发现.
虽然G/G在轻度COVID-19中很常见 (P=0.684),但在TLR7基因型和性别 (P=0.024) 之间观察到显著的关联,男性主要患有G/G,女性G/A.
在G/G基因型和肺炎之间发现了显著的关联 (P=0.023).
与女性相比,男性的严重感染率 (68%) 和死亡率 (26.4%) 较高.

结论:

一种新的TLR7变种,rs864058"G/A",在与COVID-19相关的巴基斯坦人口中被发现.
男人似乎面临更高的COVID-19发病率和死亡率风险.
建议进行进一步的大规模研究,以验证这些发现,并探索TLR7基因型在COVID-19中的影响.

关键词:

在 COVID-19 疫情中,这是一个PCRPCR.RNA病毒的RNA病毒在TLR7中,收费类受体的收费类受体.它们是mRNARNA.多形态主义的多态主义.风险因素风险因素一个单核酸的核酸.

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