高剂量的辐射会导致耳免疫应激和神经感官听力损失
Mengwen Shi1, Ye Wang2, Huiwen Yang1
1Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Heliyon
|September 23, 2024
概括
高剂量放射治疗导致小鼠急性神经感官听力损失 (SNHL),与螺旋质神经元损伤有关,而不是毛细胞损失. 内耳巨细胞的减少和免疫系统的变化可能在辐射引起的听力损失中起作用.
科学领域:
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
- 辐射瘤学 辐射瘤学
- 免疫学 免疫学 免疫学
背景情况:
- 治疗头部和部癌症的放射疗法可能会导致神经感官听力损失 (SNHL).
- 辐射对内耳的免疫微环境和感官神经上皮质的影响尚不清楚.
- 研究辐射对耳结构和巨细胞的影响对于理解SNHL机制至关重要.
研究的目的:
- 在高剂量辐射后,研究内耳的耳形态和巨细胞变化.
- 评估辐射对听觉通路的功能影响.
- 为了探索炎症调解者的表达在尾后辐射.
主要方法:
- 鼠标的头部用30GyX射线进行辐射;样本在第1,7和第10天收集.
- 听觉脑干反应评估了听觉功能.
- 血氧氨基和免疫光染色检查了毛细胞,螺旋质神经元 (SGN) 和巨细胞.
- 定量实时RT-PCR检测到耳组织中的炎症媒介表达.
主要成果:
- 在一次高剂量的辐射后,没有观察到显著的毛细胞损失.
- 小鼠在第10天就出现听力损失,SGN缩和神经丝表达减少.
- 内耳巨细胞数量随着时间的推移而减少.
- 耳炎症因子和化学因子在辐射后的第一天显示过渡性上调.
结论:
- 高剂量辐射诱导急性SNHL与脱发无关,可能是由于SGN损伤.
- 辐射诱导的SNHL与减少的耳巨细胞和改变的免疫微环境有关.
- 需要进一步的研究来阐明巨细胞减少和辐射诱导的SNHL之间的关系.
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