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在怀疑感染的患者中,元基因组测序的诊断性能:一项大规模的回顾性研究

Ziyang Li^1,2, Li Tan^1,2, Jialiang Zhang^1,2

¹Center for Clinical Molecular Diagnostics, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Frontiers in cellular and infection microbiology

|September 23, 2024

概括

与传统培养方法相比,甲基因组下一代测序 (mNGS) 对传染病具有更高的诊断价值和临床接受性. 通过mNGS分析的配对血液和呼吸道样本有助于病原体的识别.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
基因组学就是基因组学.

背景情况:

下一代元基因组测序 (mNGS) 越来越多地用于传染病 (ID) 中的病原体识别.
对于mNGS的诊断实用性和临床接受性,特别是对组样本的诊断实用性,需要对已建立的方法进行进一步的研究.
基于文化的方法仍然是标准的,但在灵敏度和检测范围方面可能存在局限性.

研究的目的:

评估对象样本mNGS对应培养的诊断性能和临床接受性,用于在疑似感染中检测病原体.
为了比较mNGS和培养试验的灵敏度和阳性率.
通过使用mNGS,研究对血和呼吸道样本之间的病原体检测一致性.

主要方法:

追溯分析361名疑似感染的患者.
与临床诊断对抗mNGS和培养试验结果的比较.
用mNGS分析的53个配对的血液和呼吸道样本中的病原体一致性分析.

主要成果:

mNGS在62.04%的样本中发现了病原体,明显高于培养 (33.18%).
与培养 (56.72%) 相比,mNGS表现出较高的灵敏度 (89.08%),相对于临床诊断.
mNGS的临床接受率明显高于培养.
通过mNGS分析的配对血液和呼吸道样本经常显示出共享的,部分匹配的病原体.

结论:

与传统培养方法相比,mNGS在诊断感染方面具有更高的灵敏度和临床接受度.
配对的血液和呼吸道mNGS分析可以有效地帮助识别病原体,特别是呼吸道感染.
这些发现支持在传染病的临床诊断中更广泛地采用mNGS.

关键词:

临床诊断临床诊断临床诊断传染病是一种传染性疾病.下一代元基因组测序的下一代测序病原体是一种病原体.灵敏度灵敏度灵敏度灵敏度灵敏度

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