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América Selene Gaona-Mendoza^1,2, Julio Armando Massange-Sánchez³, José Eleazar Barboza-Corona^1,2

¹Posgrado en Biociencias, Universidad de Guanajuato, Campus Irapuato-Salamanca, Carretera Irapuato Silao Km 9, 36500, Irapuato, Guanajuato, Mexico.

Molecular biotechnology

|September 23, 2024

概括

在Lactococcus lactis中,对表达外来蛋白质至关重要. 虽然一些基因表达高,但只有像mCherry-O这样的优化基因才能产生可检测的蛋白质,这凸显了需要仔细的基因适应的需要.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

乳球菌 (Lactococcus lactis) 广泛用于发酵食品和异质蛋白质生产.
它的Nisin控制的基因表达系统提供了安全性和多功能性.
在L. lactis中基因组GC含量较低可以限制外来蛋白质的产生.

研究的目的:

为了研究编码子优化对L. lactis.光记者蛋白表达的影响.
为了比较非优化 (gfp,rfp,mcherry) 和代优化 (mcherry-O) 基因的表达.
分析基因表达指标和蛋白质合成之间的相关性.

主要方法:

编码体适应指数 (CAI) 的量化,编码体有效数 (Enc) 和关氨酸-氨酸百分比 (% GC).
基因表达水平和通过SDS-PAGE和 lysate可视化检测蛋白质的比较.
评估光记者蛋白质的生产.

主要成果:

与非优化变体不同的是,可登优化的mCherry-O蛋白被成功检测出来.
高表达的gfp没有导致可检测的蛋白质.
Rfp显示了一些优化,但并没有针对L. lactis进行定制.

结论:

对于L. lactis有效的外来基因表达,全面的编码子优化至关重要.
由于RNA稳定性等因素,基因表达水平并不总是与蛋白质合成相关.
的优化显著影响了L. lactis.中的蛋白质检测能力和蛋白质生产.

关键词:

乳球球菌 (lactococcus lactis) 是一种乳球菌.科登使用科登使用光蛋白质是一种光蛋白质.基因表达基因表达基因表达

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