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相关概念视频

RNA Structure01:23

RNA Structure

71.2K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
71.2K
Nucleic Acid Structure01:25

Nucleic Acid Structure

6.0K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
6.0K
Nucleic Acids02:43

Nucleic Acids

43.9K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
43.9K
RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
RNA Stability01:53

RNA Stability

33.4K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.4K
Types of RNA01:20

Types of RNA

5.7K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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相关实验视频

Updated: Jun 12, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

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对于RNA-RNA相互作用的序列设计.

Maria Waldl1,2,3, Hua-Ting Yao1, Ivo L Hofacker4

  • 1Department of Theoretical Chemistry, University of Vienna, Vienna, Austria.

Methods in molecular biology (Clifton, N.J.)
|September 23, 2024
PubMed
概括
此摘要是机器生成的。

这项研究引入了一个用于设计相互作用并调节基因表达的RNA序列的计算框架. 该方法优化RNA结构用于生物技术应用,包括通过特定的RNA-RNA相互作用控制基因表达.

关键词:
的RNA折叠动力学.的RNA序列设计.结构 RNA 结构 RNA 结构RNA-RNA 相互作用

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

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相关实验视频

Last Updated: Jun 12, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

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科学领域:

  • 计算生物学 计算生物学
  • 合成生物学 合成生物学
  • 生物技术是生物技术.

背景情况:

  • 调节性RNA通过RNA-RNA相互作用来控制基因表达.
  • 设计具有特定结构性质的RNA序列在计算上具有挑战性.
  • 通过RNA-RNA相互作用控制基因表达具有重要的生物技术潜力.

研究的目的:

  • 展示使用红外框架来设计相互作用的RNA序列.
  • 设计人工未翻译区域 (UTR),通过与特定小RNA (sRNA) 相互作用来调节基因表达.
  • 将热力学和动力学折叠特征集成到RNA设计过程中.

主要方法:

  • 利用红外计算框架进行序列设计.
  • 框架内内置的设计约束,质量措施和成本函数.
  • 使用RRIkinDP工具估计并包括动力折叠特征.
  • 在 Jupyter 笔记本中用 Python 代码开发了一个逐步协议.

主要成果:

  • 成功设计了人工UTR来控制由DsrAsRNA调节的基因表达.
  • 证明了RNA设计中热力学和动力学折叠特征的相关性.
  • 展示了动力折叠数据与设计成本函数的整合.

结论:

  • 红外框架对于设计具有所需调节功能的相互作用RNA序列是有效的.
  • 开发的协议为RNA序列设计提供了一种灵活的方法,可适应各种生物技术和生物医学应用.
  • 整合动力折叠特性可以增强功能调节RNA的设计.