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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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相关实验视频

Updated: Jun 12, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

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造型探测用于选计算机设计的RNA.

Pierre Hardouin1, Francois-Xavier Lyonnet du Moutier1, Bruno Sargueil2

  • 1CiTCOM, Cibles Thérapeutiques et Conception de Médicaments, UMR8038 CNRS, Université Paris Cité, Paris, France.

Methods in molecular biology (Clifton, N.J.)
|September 23, 2024
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种精简的工作流和软件工具,以快速选数千个设计的RNA序列. 这通过简化实验分析来加速合成生物学和基于RNA的疗法的RNA设计.

关键词:
化学探测 化学探测 化学探测足迹 足迹是指足迹的存在.这就是IPANEMAP.预测RNA结构 预测RNA结构

更多相关视频

RNA Secondary Structure Prediction Using High-throughput SHAPE
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RNA Secondary Structure Prediction Using High-throughput SHAPE

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An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
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An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

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相关实验视频

Last Updated: Jun 12, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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RNA Secondary Structure Prediction Using High-throughput SHAPE
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RNA Secondary Structure Prediction Using High-throughput SHAPE

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An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
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An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

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科学领域:

  • 合成生物学 合成生物学
  • 在RNA治疗方面,RNA疗法.
  • 计算生物学 计算生物学

背景情况:

  • 对于合成生物学和基于RNA的疗法来说,RNA设计至关重要,但目前的方法依赖于低效的试错.
  • 高通量RNA结构探测 (例如,SHAPE-MaP) 可以快速进行大规模分析,但产生复杂的数据.
  • 对细微的序列差异分析众多实验结果是耗时且具有挑战性的.

研究的目的:

  • 开发一个集成的实验和分析工作流程,以有效选众多设计的RNA序列.
  • 为了简化和加快高通量RNA结构探测数据的分析.
  • 为RNA设计和评估提供一个用户友好的软件解决方案.

主要方法:

  • 为高通量RNA序列查实施一种新的实验工作流程.
  • 开发用于定制数据分析的"shapemap工具"软件套件.
  • 将化学探测数据与用于序列评估的计算分析集成在一起.

主要成果:

  • 拟议的工作流成功地同时对数千个设计的RNA序列进行选.
  • "形状图工具"软件套件为分析复杂的RNA结构探测数据提供了定制解决方案.
  • 显著减少评估实验结果所需的时间和精力.

结论:

  • 开发的工作流和软件工具加速了RNA设计管道.
  • 这种方法促进了合成生物学和基于RNA的治疗开发的进步.
  • 这些工具为分析大规模RNA结构数据提供了可扩展的解决方案.