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相关概念视频

Ribozymes02:47

Ribozymes

11.2K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
11.2K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

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Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

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Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.4K
Synthetic Biology02:55

Synthetic Biology

4.7K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
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相关实验视频

Updated: Jun 12, 2025

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

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使用稀疏的共同进化模型生成人工 ribozymes.

Francesco Calvanese1,2, Martin Weigt1, Philippe Nghe3

  • 1Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, Laboratoire de Biologie Computationnelle et Quantitative - LCQB Paris, Paris, France.

Methods in molecular biology (Clifton, N.J.)
|September 23, 2024
PubMed
概括
此摘要是机器生成的。

生成模型探索了超越自然进化的广RNA序列空间. 直接合分析 (DCA) 创建了新的旋转核糖酶,并预测了突变效应.

关键词:
人工RNA序列的测序.DCA DCA 的意思是什么?直接合分析直接合分析生成型模型是一种生成型模型.突变效应的影响.

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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

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相关实验视频

Last Updated: Jun 12, 2025

Substrate Generation for Endonucleases of CRISPR/Cas Systems
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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

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科学领域:

  • 计算生物学 计算生物学
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 与巨大的潜在RNA序列空间相比,RNA序列数据集是有限的.
  • 自然进化只探索了一小部分可能的RNA序列.
  • 生成模型提供了一种强大的方法来探索未知序列可变性.

研究的目的:

  • 将基于直接合分析 (DCA) 的生成模型应用于旋转核糖酶RNA家族.
  • 为了产生人造的旋转 ribozymes.
  • 设计功能突变并预测突变效应.

主要方法:

  • 使用基于直接合分析 (DCA) 的生成模型.
  • 将该模型应用于旋转核糖酶RNA家族.
  • 专注于生成新的序列,设计突变和预测效应.

主要成果:

  • 成功生成了人造的旋转 ribozymes.
  • 确定了天然野生类型序列的潜在功能突变.
  • 证明了模型在预测突变效应方面的能力.

结论:

  • 生成模型,特别是基于DCA的模型,对于探索RNA序列空间是有效的.
  • 这种方法扩大了已知的旋转核糖酶谱.
  • 便于设计具有潜在功能性质的新型RNA分子.