适应HistoEnder,一个开源的3D打印机用于自动传输电子显微镜网格染色的自动传输电子显微镜
1Morphology Core, Schepens Eye Research Institute of Mass Eye and Ear Boston, Boston, MA, USA.
Journal of histotechnology
|September 23, 2024
概括
在HistoEnder 3D打印机自动化传输电子显微镜 (TEM) 网格染色. 这种低成本,开源的适应实现了与生物样本手工方法相同的结果.
科学领域:
- 显微镜的使用方法
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 传统传输电子显微镜 (TEM) 网格染色是一种手动,耗时的过程.
- 自动染色方法可以提高TEM样品准备的效率和可重复性.
- 开源3D打印机HistoEnder最初是为组织学幻灯片染色而设计的.
研究的目的:
- 适应HistoEnder 3D打印机用于传输电子显微镜网格的自动批次染色.
- 评估这种适应TEM样本准备系统的有效性和成本效益.
主要方法:
- 该HistoEnder装置是3D打印,并根据所提供的细节组装.
- G-code编程是为3D打印机的自动操作而开发的.
- 该系统被编程为使用酸,水冲洗和染料对多达20个网格进行切割后染色.
主要成果:
- 适应的HistoEnder成功地自动化了传输电子显微镜网的批次染色.
- 自动化染色过程包括乌兰酸,蒸水冲洗和污点.
- 使用HistoEnder获得的传输电子显微镜结果与手动染色方法的结果相同.
结论:
- HistoEnder提供了一个低成本的开源解决方案,用于自动化传输电子显微镜网格染色.
- 这种适应提供了与手工方法相同的染色质量,并具有自动化的好处.
- "HistoEnder"代表了在可访问和高效的TEM样本准备方面取得的重大进展.
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