设计和优化含有胰岛素的基托桑涂层固体脂质纳米颗粒,以提高肠道透性,使用 piperine
Indu Raghunath1, Marina Koland1, C Sarathchandran2
1Nitte (Deemed to be University), NGSM Institute of Pharmaceutical Sciences, Department of Pharmaceutics, Mangalore, Karnataka 575018, India.
International journal of biological macromolecules
|September 23, 2024
概括
皮佩林可以增强素涂层固体脂质纳米颗粒 (Ch-In-SLNs) 的肠道胰岛素透性. 这种配方显示了持续的胰岛素释放和对胃酶的保护,改善了口服胰岛素的输送潜力.
科学领域:
- 纳米技术 纳米技术
- 药品制造 药品制造 药品制造
- 生物材料是一种生物材料.
背景情况:
- 口服胰岛素因酶降解和透性差而面临挑战.
- 固体脂质纳米颗粒 (SLN) 为治疗性蛋白质提供了一个有前途的载体系统.
- 基托桑涂层可以增强纳米粒子粘粘和药物释放.
研究的目的:
- 为了优化素涂层的胰岛素载固体脂质纳米颗粒 (Ch-In-SLNs).
- 为了评估 piperine 对胰岛素通过胃肠道粘膜的透的影响.
- 评估Ch-In-SLNs在口服胰岛素输送中的体外性能.
主要方法:
- 使用甘乙烯酸,大豆莱西丁和poloxamer® 407.7的SLNs的配方.
- 使用盒式设计 (BBD) 优化Ch-In-SLNs.
- 试验室表征包括颗粒大小,PDI,泽塔潜力,关联效率和药物释放研究.
- 使用山羊肠和Caco-2细胞系进行体外透研究.
- 细胞吸收研究.
主要成果:
- 优化的Ch-In-SLN表现出最佳的粒子大小 (654.43nm),正色达电位 (+36.87 mV) 和良好的单分散性 (PDI = 0.4).
- 在模拟的胃和肠液中,持续12小时释放胰岛素,在肠液中释放量增加.
- 封装胰岛素在模拟的胃和肠液中有效地保护其免受酶分解.
- 皮佩林显著增强了山羊肠和Caco-2细胞系的胰岛素透.
- 细胞吸收研究证实了皮佩林在改善胰岛素透中的作用.
结论:
- 优化的Ch-In-SLNs提供了稳定有效的胰岛素输送系统.
- 皮佩林作为一种有效的透增强剂,通过Ch-In-SLNs传递胰岛素.
- 这种配方有可能改善口服胰岛素的生物可用性.
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