针对Wnt信号通路的天然小分子化合物抑制了HPV感染
Tao Zhang1, Ze Wang2, Munawaer Muaibati2
1Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology, 1095 JieFang Avenue, Wuhan, 430030, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen, 518057, China.
Microbial pathogenesis
|September 23, 2024
概括
高风险的人类乳头瘤病毒 (HPV) 感染是导致子宫癌的原因,可能是可治疗的. 研究人员发现,通过使用像6-甘醇这样的天然化合物准Wnt信号通路,可以抑制HPV感染.
科学领域:
- 病毒学和分子生物学.
- 癌症研究 癌症研究
- 药物发现 药物发现
背景情况:
- 高风险的人类乳头瘤病毒 (HPV) 感染是HPV相关癌症,特别是宫癌的重要风险因素.
- 目前,没有专门的抗病毒药物用于治疗HPV感染.
研究的目的:
- 为了调查Wnt标志性信号通路在HPV16感染中的参与.
- 通过准Wnt途径来识别抑制HPV16感染的天然小分子化合物.
主要方法:
- 使用特定抑制剂 (IWP-2,FH535) 抑制Wnt/β-catenin信号通路.
- 通过光显微镜和流细胞计评估HPV16-GFP伪病毒的感染性.
- 对29种FDA批准的天然化合物进行选,这些化合物针对Wnt通路.
主要成果:
- Wnt信号通路抑制剂在HaCat细胞中显示抑制HPV16-GFP伪病毒感染.
- 查确定了6-甘醇,戈西波尔,坦辛II2A和EGCG作为HPV16-GFP伪病毒感染的有效抑制剂.
- 这些化合物还抑制了HaCat细胞中的Wnt/β-catenin通路.
结论:
- 正规的Wnt信号通路在HPV感染宿主细胞中起作用.
- 天然化合物6-醇,gossypol,tanshinone II2A和EGCG通过抑制Wnt/β-catenin通路显示出作为治疗HPV感染的治疗剂的潜力.
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