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MiR-145-5p通过向KGN细胞中的SET基因来调节粒粉细胞的增殖

Gao Lingling^1,2, Yang Qingxing^1,2, Xu Jianbo^1,2

¹Department of Obstetrics and Gynecology, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Jiangsu 225001, China.

The Journal of reproduction and development

|September 23, 2024

概括

微RNA-145-5p (miR-145-5p) 通过向SET,抑制了多囊性卵巢综合征 (PCOS) 中的花状细胞增殖. 减少miR-145-5p和增加SET有助于PCOS毛囊功能障碍.

科学领域:

内分泌学在内分泌学.
分子生物学分子生物学
生殖医学生殖医学

背景情况:

微RNA-145-5p (miR-145-5p) 是一种已知的瘤抑制剂,在多囊性卵巢综合征 (PCOS) 颗粒细胞中观察到表达的减少.
多囊性卵巢综合征 (PCOS) 与颗粒细胞功能障碍和毛囊发育受损有关.

研究的目的:

为了研究miR-145-5p在调节粒粉细胞增殖中的作用.
阐明PCOS中毛囊发育障碍背后的分子机制.

主要方法:

从PCOS患者和健康对照组收集的毛囊液.
使用细胞计数工具-8和氧氨试验来评估KGN细胞增殖.
进行定量实时PCR测量miR-145-5p和SET表达.
通过生物信息分析和实验验证,确定了直接目标相互作用.

主要成果:

在PCOS粒状细胞中,miR-145-5p的表达与对照细胞相比显著较低.
SET表达与miR-145-5p水平相反相关,并在PCOS上调调节.
miR-145-5p抑制了KGN细胞的增殖,而SET则促进了它.
证实SET是miR-145-5p的直接标,其淘汰逆转了miR-145-5p抑制的作用.

结论:

miR-145-5p通过向SET. 作为颗粒细胞增殖的负调节剂.
miR-145-5p/SET通路是PCOS毛囊发育异常病理生理学的潜在贡献者.

关键词:

粒粉细胞的增殖粒粉细胞的增殖在米R-145-5p.设置设置设置

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