Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.7K
Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

7.9K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
7.9K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.0K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

2.7K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Outbreak of Ralstonia Mannitolytica Bacteremia in the Paediatric Critical Unit due to Intrinsic Contamination of Dexmedetomidine.

Indian journal of medical microbiology·2026
Same author

Anticoagulation with mechanistically distinct FXI/FXIa antibodies amrecibart (REGN9933A2) and cenvacibart (REGN7508Cat).

Blood·2026
Same author

In vitro activity of aztreonam/avibactam and comparators against Enterobacterales isolates from patients with hospital-acquired pneumonia, ventilator-associated pneumonia, and complicated intra-abdominal infections (ATLAS, 2021-2022).

The Journal of antimicrobial chemotherapy·2026
Same author

Isoform-specific cofactor recruitment through the intrinsically disordered N-terminus of p63 underlies differential transcriptional activities.

bioRxiv : the preprint server for biology·2026
Same author

An experimental approach to assess the combined effects of multiple stressors on a large vertebrate species.

Conservation biology : the journal of the Society for Conservation Biology·2026
Same author

The combined effects of multiple stressors in an endangered, long-lived species: Lessons learned and ways forward.

Ecological applications : a publication of the Ecological Society of America·2025

相关实验视频

Updated: Jun 12, 2025

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
06:56

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

13.6K

对CXCR4调制和寡合化的结构见解.

Kei Saotome1, Luke L McGoldrick2, Jo-Hao Ho3

  • 1Regeneron Pharmaceuticals, Inc., Tarrytown, NY, USA. kei.saotome@regeneron.com.

Nature structural & molecular biology
|September 23, 2024
PubMed
概括
此摘要是机器生成的。

通过CXCL12激活化基因受体CXCR4的方法详细使用了冷EM. 结构显示了对手AMD3100和抗体REGN7663的结合,以及影响CXCR4寡合化的功能.

更多相关视频

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
07:41

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

Published on: February 20, 2018

8.9K
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
08:55

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Published on: October 28, 2016

10.2K

相关实验视频

Last Updated: Jun 12, 2025

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
06:56

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

13.6K
A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
07:41

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

Published on: February 20, 2018

8.9K
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
08:55

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Published on: October 28, 2016

10.2K

科学领域:

  • 结构生物学 结构生物学
  • 分子药理学分子药理学
  • 细胞信号传输 细胞信号传输

背景情况:

  • 化学因子受体CXCR4 (C-X-C化学因子受体类型4) 在各种细胞过程中发挥着关键作用.
  • 之前的结构研究揭示了CXCR4的非活性,同位体形式,使得许多监管方面的理解不足.

研究的目的:

  • 使用冷电子显微镜研究人类CXCR4调节的结构机制.
  • 为了阐明配体CXCL12,对手AMD3100和抗体REGN7663.3的结合方式.
  • 探索CXCR4寡合化的结构基础及其功能影响.

主要方法:

  • 使用冷电子显微镜 (cryo-EM) 来确定CXCR4.4的高分辨率结构.
  • 对受体的连接体和寡合体状态的分析.

主要成果:

  • 化学因子CXCL12 (C-X-C动机化学因子连接体12) 通过将其N端插入orthosteric口袋来激活CXCR4.
  • 抗体AMD3100通过静电相互作用与受体的跨膜束内的酸性残留结合.
  • 抗体REGN7663与细胞外面结合,并插入orthosteric口袋.
  • 冷-EM揭示了CXCR4的三元和四元组合,显示出不同的亚单元构造.

结论:

  • CXCR4的结构和功能是由联体结合,抗体和抗体调节的.
  • 将CXCR4氧化成三聚体和四聚体形式影响受体构造和功能,这表明了全聚体调节.
  • 这些发现为化学因受体结构生物学和潜在的治疗向提供了关键的见解.