Pyricularia oryzae通过非挥发性性代谢物增强了Streptomyces griseus的生长
Risa Sugiura1, Takayuki Arazoe1, Takayuki Motoyama2
1Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda-shi, Japan.
Environmental microbiology reports
|September 24, 2024
概括
米爆真菌Pyricularia oryzae通过释放性化合物,可能是多氨酸,到生长介质中来促进Streptomyces griseus的生长. 这种相互作用凸显了微生物群落中的化学信号.
科学领域:
- 微生物学 微生物学
- 植物病理学 植物病理学
- 生物化学 生物化学
背景情况:
- 微生物相互作用在各种生态系统中至关重要.
- 化学信号介导微生物之间的物种间通信.
- 了解这些相互作用可以揭示新的生化途径.
研究的目的:
- 为了研究Pyricularia oryzae对Streptomyces griseus生长的影响.
- 阐明观察到的增长促进背后的机制.
- 为了识别参与相互作用的化学化合物.
主要方法:
- 在土豆德克斯特酸 (PDA) 上共同培养S. griseus和P. oryzae.
- 评估化学信号的增长促进和扩散.
- 使用红色指示器监测pH值变化.
- 使用DL-α-Difluoromethylornithine来抑制聚胺生物合成.
主要成果:
- 在直接的共同培养中,P. oryzae显著增强了S. griseus的生长.
- 通过可扩散,非挥发性化合物来促进生长.
- P. oryzae增加了周围环境的pH值,这与S. griseus的生长相关.
- DL-α-Difluoromethylornithine消除了pH值的增加和增长促进,这表明聚胺参与其中.
结论:
- Pyricularia oryzae产生性化合物,可能是多氨酸,促进Streptomyces griseus的生长.
- 相互作用是由生长介质的pH值变化介导的.
- 这项研究揭示了植物病原菌和土壤细菌之间的新型化学通信途径.
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