基 - 聚二松复合凝的合成,特征和骨质生成特性
Kavipriya Murugaiyan1, Arthi Chandramouli1, Jayakumar Rangasamy1
1Polymeric Biomaterials Lab, School of Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi, 682041, India.
Chemistry, an Asian journal
|September 24, 2024
概括
一种新的基-多聚二松复合凝显示了组织工程的前景. 这种生物相容的水凝支持细胞附着,增强骨形成,使其成为骨组织再生应用的潜在候选者.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 由于可调节性质和生物相容性,水凝被广泛用于生物医学应用.
- 素 (Ch) 模仿自然的细胞外基质,而聚二松 (PDO) 则提供机械强度和生物相容性.
- 结合Ch和PDO,产生一种复合凝,具有用于组织工程的增强特性.
研究的目的:
- 开发和描述一种新的奇-多-二松) 复合凝.
- 评估凝的物理特性,包括凝强度,胀,降解和可注射性.
- 使用牙囊干细胞 (DFSCs) 评估Ch-PDO凝的生物相容性,细胞粘附性和骨质生成潜力.
主要方法:
- 使用简单的再生化学合成的Ch-PDO复合凝.
- 通过里埃转换红外光谱学 (FT-IR) 和扫描电子显微镜 (SEM) 进行表征.
- 评估物理特性 (凝强度,胀,降解,可注射性,血液相容性) 和生物性能 (生物相容性,细胞粘附性,骨质矿化,基因表达).
主要成果:
- Ch-PDO凝表现出改善的凝强度,良好的膨胀能力和受控的降解.
- 复合凝表现出良好的注射性,具有剪切稀释特性和血液相容性.
- 生物相容性和DFSC的附着性得到证实;Ch-PDO凝增强了骨质生成矿化,RUNX2和OPN表达,表明优异的骨质生成活性.
结论:
- 开发的奇-多聚二松复合凝是生物相容的,具有有利的物理性能.
- 凝支持牙毛囊干细胞的附着,并促进骨质分化.
- 这种Ch-PDO水凝具有重要的骨组织工程应用潜力.
相关概念视频
01:55Bone Structure
Within the skeletal system, the structure of a bone, or osseous tissue, can be exemplified in a long bone, like the femur, where there are two types of osseous tissue: cortical and cancellous.
01:27Growth of Cartilage and Bone Tissue
Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
01:23Bone as Supporting Connective Tissue
Bone tissue forms the internal skeleton of vertebrate animals, providing structure to the body.
Bone Matrix
Bone, or osseous tissue, is a connective tissue that has a large amount of two different types of matrix material. The organic matrix is similar to the matrix material found in other connective tissues, including some amount of collagen and elastic fibers. This gives strength and flexibility to the tissue. The inorganic matrix consists of mineral salts— mostly calcium salts— that give the...
Bone Matrix
Bone, or osseous tissue, is a connective tissue that has a large amount of two different types of matrix material. The organic matrix is similar to the matrix material found in other connective tissues, including some amount of collagen and elastic fibers. This gives strength and flexibility to the tissue. The inorganic matrix consists of mineral salts— mostly calcium salts— that give the...
01:18The Bone Matrix
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in acid or...
01:27Compact Bone
Most bones contain compact and spongy osseous tissue, but their distribution and concentration vary based on the bone's overall function.
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
01:09Spongy Bone
All bones comprise an outer layer of compact bone, and an interior made up of spongy bone tissue, also called cancellous or trabecular bone. In long bones, spongy bone tissue is mainly found in the interior of the epiphyses (broad ends of the bone).
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...