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Updated: Jun 12, 2025

Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
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Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells

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在艾滋病毒基因组中毫不含糊地检测LTR-III G-四重体,使用定制的化探针测试

Sumon Pratihar1, Vasudhar Bhat S V1, Krithi K Bhagavath1

  • 1Bioorganic Chemistry Laboratory, New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR), Jakkur, Bengaluru, 560064 Karnataka, India.

Analytical chemistry
|September 24, 2024
PubMed
概括

这项研究为检测人类免疫缺陷病毒 (HIV) 提供了一个新的诊断平台. 它使用G-quadruplex (GQ) DNA结构和光探针来准确检测艾滋病毒.

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Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection07:24

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

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High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing07:18

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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