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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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相关实验视频

Updated: Jun 12, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

用小角度散射进行结构分析的RNA:RNA复合物的选择性化.

Aldrex Munsayac¹, Wellington C Leite², Jesse B Hopkins³

¹Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA.

bioRxiv : the preprint server for biology

|September 24, 2024

概括

这项研究引入了一种新的散射方法来分析RNA:RNA复杂结构. 该技术将计算建模与实验数据相结合,以揭示RNA组件的结构变化.

关键词:

艾滋病毒-1二分化启动部位 (DIS)结构 RNA 结构 RNA 结构 RNA结构建模RNA结构建模 RNA:RNA复合体中的RNA.整合性结构生物学整合性结构生物学同位素标签的标签微角X射线散射 (SAXS) 是一种微角X射线散射技术.微角中子散射 (SANS) 是一种微角中子散射.

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RNA Secondary Structure Prediction Using High-throughput SHAPE

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相关实验视频

Last Updated: Jun 12, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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Published on: December 9, 2022

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科学领域:

结构生物学结构生物学
生物物理学的生物物理.
分子生物学分子生物学

背景情况:

无蛋白RNA:RNA复合体对于生物过程至关重要,但在结构上不足.
了解RNA:RNA复杂动态对于破译它们的功能至关重要.

研究的目的:

开发和演示一种联合小角度X射线和中子散射 (SAXS/SANS) 方法,用于查询RNA:RNA复杂结构.
用实验散射数据验证和完善像AlphaFold 3这样的计算模型.

主要方法:

利用SAXS来确定单个RNA及其复合物的溶液结构.
采用具有同位素标记和对比匹配 (CM) 的SANS来探测选择性降解的RNA:RNA复合体.
与实验SAXS/SANS数据集成的化建模.

主要成果:

使用SAXS.测量了自由RNA和整体RNA:RNA复合物的溶液结构.
通过CM和同位素标记来分析复合体内的结合RNA结构.
展示了实验数据如何验证和改进RNA:RNA复合体的AlphaFold 3预测.

结论:

结合SAXS/SANS方法,与化模型一起,有效地分析RNA:RNA复合体的构造变化.
这种综合策略增强了对功能组件内的RNA结构的理解.
该方法为研究溶液中的动态RNA结构提供了一个强大的工具.